Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme

M. Javed Iqbal, Maryam Majeed, Maheen Humayun, David A. Lightfoot, Ahmed Afzal

Research output: Contribution to journalArticle

Abstract

Sudden death syndrome (SDS) is a complex of two diseases of soybean (Glycine max), caused by the soil borne pathogenic fungus Fusarium virguliforme. The root rot and leaf scorch diseases both result in significant yield losses worldwide. Partial SDS resistance has been demonstrated in multiple soybean cultivars. This study aimed to highlight proteomic changes in soybean roots by identifying proteins which are differentially expressed in near isogenic lines (NILs) contrasting at the Rhg1/Rfs2 locus for partial resistance or susceptibility to SDS. Two-dimensional gel electrophoresis resolved approximately 1000 spots on each gel; 12 spots with a significant (P < 0.05) difference in abundance of 1.5-fold or more were picked, trypsin-digested, and analyzed using quadruple time-of-flight tandem mass spectrometry. Several spots contained more than one protein, so that 18 distinct proteins were identified overall. A functional analysis performed to categorize the proteins depicted that the major pathways altered by fungal infection include disease resistance, stress tolerance, and metabolism. This is the first report which identifies proteins whose abundances are altered in response to fungal infection leading to SDS. The results provide valuable information about SDS resistance in soybean plants, and plant partial resistance responses in general. More importantly, several of the identified proteins could be good candidates for the development of SDS-resistant soybean plants.

Original languageEnglish (US)
Pages (from-to)1657-1674
Number of pages18
JournalApplied Biochemistry and Biotechnology
Volume180
Issue number8
DOIs
StatePublished - Dec 1 2016

Fingerprint

Fusarium
Sudden Death
Soybeans
Proteomics
Proteins
Mycoses
Gels
Functional analysis
Disease Resistance
Electrophoresis, Gel, Two-Dimensional
Tandem Mass Spectrometry
Electrophoresis
Fungi
Metabolism
Glycine
Trypsin
Mass spectrometry
Amino acids
Soil
Soils

Keywords

  • 2D gel electrophoresis
  • Proteomics
  • Rhg1/Rfs2 locus
  • Soybean
  • Sudden death syndrome

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme. / Iqbal, M. Javed; Majeed, Maryam; Humayun, Maheen; Lightfoot, David A.; Afzal, Ahmed.

In: Applied Biochemistry and Biotechnology, Vol. 180, No. 8, 01.12.2016, p. 1657-1674.

Research output: Contribution to journalArticle

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