Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains

Taejoon Kwon, Holly K. Huse, Christine Vogel, Marvin Whiteley, Edward M. Marcotte

Research output: Contribution to journalArticle

Abstract

Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here, we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we found that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that the magnitude and direction of the effect of protein abundance regulation occurring after the setting of mRNA levels is conserved between bacterial strains and is important for explaining the discordance between mRNA and protein abundances.

Original languageEnglish (US)
Pages (from-to)2370-2380
Number of pages11
JournalJournal of Proteome Research
Volume13
Issue number5
DOIs
StatePublished - May 2 2014

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Pseudomonas aeruginosa
Messenger RNA
Genes
Proteins
Firearms
Proteome
Microarrays
Microbial Drug Resistance
Transcriptome
Proteomics
Environmental impact
Virulence
Anti-Bacterial Agents
Growth
Experiments

Keywords

  • proteomics
  • Pseudomonas aeruginosa
  • Transcriptomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains. / Kwon, Taejoon; Huse, Holly K.; Vogel, Christine; Whiteley, Marvin; Marcotte, Edward M.

In: Journal of Proteome Research, Vol. 13, No. 5, 02.05.2014, p. 2370-2380.

Research output: Contribution to journalArticle

Kwon, Taejoon ; Huse, Holly K. ; Vogel, Christine ; Whiteley, Marvin ; Marcotte, Edward M. / Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 5. pp. 2370-2380.
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