Protein S-thiolation and depletion of intracellular glutathione in skin fibroblasts exposed to various sources of oxidative stress

S. Salman Ashraf, Sehamuddin Galadari, Mahendra Patel

    Research output: Contribution to journalArticle

    Abstract

    It is well established that oxidative stress plays a central role in the onset and progression of over a 100 different diseases. Recently, a growing body of evidence has shown that chemicals/agents as diverse as aromatic compounds, UV radiation and redox-active metals also generate oxy-radicals in vivo and lead to cellular oxidative stress. In this study we have used mouse skin fibroblasts to study the effects of oxidative stress caused by organic aromatic compounds (xylene), UV radiation and redox-active metals. Specifically, we tested the effect of these treatments on intracellular GSH levels, as well as on protein S-thiolation. We show that acute exposure of these diverse set of conditions cause dramatic depletion of the intracellular GSH pool in mouse skin fibroblast cells. We also found evidence of synergistic effects of combined exposure to different sources of oxidative stress. Furthermore, we also found that these treatments also caused significant S-thiolation (protein mixed-disulfide formation) of a 70 kDa cytosolic protein.

    Original languageEnglish (US)
    Pages (from-to)75-79
    Number of pages5
    JournalEnvironmental Toxicology and Pharmacology
    Volume22
    Issue number1
    DOIs
    StatePublished - Jul 1 2006

    Fingerprint

    Oxidative stress
    Protein S
    Fibroblasts
    Glutathione
    Skin
    Oxidative Stress
    Aromatic compounds
    Ultraviolet radiation
    Oxidation-Reduction
    Metals
    Radiation
    Xylenes
    Disulfides
    Cells
    Proteins

    Keywords

    • Glutathione
    • S-thiolation
    • Skin fibroblasts

    ASJC Scopus subject areas

    • Toxicology
    • Pharmacology
    • Health, Toxicology and Mutagenesis

    Cite this

    Protein S-thiolation and depletion of intracellular glutathione in skin fibroblasts exposed to various sources of oxidative stress. / Ashraf, S. Salman; Galadari, Sehamuddin; Patel, Mahendra.

    In: Environmental Toxicology and Pharmacology, Vol. 22, No. 1, 01.07.2006, p. 75-79.

    Research output: Contribution to journalArticle

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