Protein Engineered Triblock Polymers Composed of Two SADs: Enhanced Mechanical Properties and Binding Abilities

Andrew J. Olsen, Priya Katyal, Jennifer S. Haghpanah, Matthew B. Kubilius, Ruipeng Li, Nicole L. Schnabel, Sean C. O'Neill, Yao Wang, Min Dai, Navjot Singh, Raymond S. Tu, Jin Montclare

Research output: Contribution to journalArticle

Abstract

Recombinant methods have been used to engineer artificial protein triblock polymers composed of two different self-assembling domains (SADs) bearing one elastin (E) flanked by two cartilage oligomeric matrix protein coiled-coil (C) domains to generate CEC. To understand how the two C domains improve small molecule recognition and the mechanical integrity of CEC, we have constructed CL44AECL44A, which bears an impaired CL44A domain that is unstructured as a negative control. The CEC triblock polymer demonstrates increased small molecule binding and ideal elastic behavior for hydrogel formation. The negative control CL44AECL44A does not exhibit binding to small molecule and is inelastic at lower temperatures, affirming the favorable role of C domain and its helical conformation. While both CEC and CL44AECL44A assemble into micelles, CEC is more densely packed with C domains on the surface enabling the development of networks leading to hydrogel formation. Such protein engineered triblock copolymers capable of forming robust hydrogels hold tremendous promise for biomedical applications in drug delivery and tissue engineering.

Original languageEnglish (US)
Pages (from-to)1552-1561
Number of pages10
JournalBiomacromolecules
Volume19
Issue number5
DOIs
StatePublished - May 14 2018

Fingerprint

Hydrogels
Polymers
Hydrogel
Proteins
Mechanical properties
Molecules
Bearings (structural)
Cartilage Oligomeric Matrix Protein
Elastin
Cartilage
Micelles
Drug delivery
Tissue engineering
Block copolymers
Conformations
Engineers
Temperature

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Protein Engineered Triblock Polymers Composed of Two SADs : Enhanced Mechanical Properties and Binding Abilities. / Olsen, Andrew J.; Katyal, Priya; Haghpanah, Jennifer S.; Kubilius, Matthew B.; Li, Ruipeng; Schnabel, Nicole L.; O'Neill, Sean C.; Wang, Yao; Dai, Min; Singh, Navjot; Tu, Raymond S.; Montclare, Jin.

In: Biomacromolecules, Vol. 19, No. 5, 14.05.2018, p. 1552-1561.

Research output: Contribution to journalArticle

Olsen, AJ, Katyal, P, Haghpanah, JS, Kubilius, MB, Li, R, Schnabel, NL, O'Neill, SC, Wang, Y, Dai, M, Singh, N, Tu, RS & Montclare, J 2018, 'Protein Engineered Triblock Polymers Composed of Two SADs: Enhanced Mechanical Properties and Binding Abilities', Biomacromolecules, vol. 19, no. 5, pp. 1552-1561. https://doi.org/10.1021/acs.biomac.7b01259
Olsen, Andrew J. ; Katyal, Priya ; Haghpanah, Jennifer S. ; Kubilius, Matthew B. ; Li, Ruipeng ; Schnabel, Nicole L. ; O'Neill, Sean C. ; Wang, Yao ; Dai, Min ; Singh, Navjot ; Tu, Raymond S. ; Montclare, Jin. / Protein Engineered Triblock Polymers Composed of Two SADs : Enhanced Mechanical Properties and Binding Abilities. In: Biomacromolecules. 2018 ; Vol. 19, No. 5. pp. 1552-1561.
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