Protein-engineered nanoscale micelles for dynamic 19 F magnetic resonance and therapeutic drug delivery

Lindsay K. Hill, Joseph A. Frezzo, Priya Katyal, Dung Minh Hoang, Zakia Ben Youss Gironda, Cynthia Xu, Xuan Xie, Erika Delgado-Fukushima, Youssef Z. Wadghiri, Jin Montclare

Research output: Contribution to journalArticle

Abstract

Engineered proteins provide an interesting template for designing fluorine-19 ( 19 F) magnetic resonance imaging (MRI) contrast agents, yet progress has been hindered by the unpredictable relaxation properties of fluorine. Herein, we present the biosynthesis of a protein block copolymer, termed "fluorinated thermoresponsive assembled protein" (F-TRAP), which assembles into a monodisperse nanoscale micelle with interesting 19 F NMR properties and the ability to encapsulate and release small therapeutic molecules, imparting potential as a diagnostic and therapeutic (theranostic) agent. The assembly of the F-TRAP micelle, composed of a coiled-coil pentamer corona and a hydrophobic, thermoresponsive elastin-like polypeptide core, results in a drastic depression in spin-spin relaxation (T 2 ) times and unaffected spin-lattice relaxation (T 1 ) times. The nearly unchanging T 1 relaxation rates and linearly dependent T 2 relaxation rates have allowed for detection via zero echo time 19 F MRI, and the in vivo MR potential has been preliminarily explored using 19 F magnetic resonance spectroscopy (MRS). This fluorinated micelle has also demonstrated the ability to encapsulate the small-molecule chemotherapeutic doxorubicin and release its cargo in a thermoresponsive manner owing to its inherent stimuli-responsive properties, presenting an interesting avenue for the development of thermoresponsive 19 F MRI/MRS-traceable theranostic agents.

Original languageEnglish (US)
JournalACS Nano
DOIs
StatePublished - Jan 1 2019

Fingerprint

Micelles
Magnetic resonance
Drug delivery
magnetic resonance
delivery
micelles
drugs
Magnetic resonance spectroscopy
proteins
Proteins
Fluorine
magnetic resonance spectroscopy
Imaging techniques
fluorine
elastin
Elastin
Molecules
biosynthesis
cargo
Spin-lattice relaxation

Keywords

  • F MRI
  • drug delivery
  • micelle
  • protein engineering
  • self-assembly
  • theranostic
  • thermoresponsiveness

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Protein-engineered nanoscale micelles for dynamic 19 F magnetic resonance and therapeutic drug delivery . / Hill, Lindsay K.; Frezzo, Joseph A.; Katyal, Priya; Hoang, Dung Minh; Ben Youss Gironda, Zakia; Xu, Cynthia; Xie, Xuan; Delgado-Fukushima, Erika; Wadghiri, Youssef Z.; Montclare, Jin.

In: ACS Nano, 01.01.2019.

Research output: Contribution to journalArticle

Hill, LK, Frezzo, JA, Katyal, P, Hoang, DM, Ben Youss Gironda, Z, Xu, C, Xie, X, Delgado-Fukushima, E, Wadghiri, YZ & Montclare, J 2019, ' Protein-engineered nanoscale micelles for dynamic 19 F magnetic resonance and therapeutic drug delivery ', ACS Nano. https://doi.org/10.1021/acsnano.8b07481
Hill, Lindsay K. ; Frezzo, Joseph A. ; Katyal, Priya ; Hoang, Dung Minh ; Ben Youss Gironda, Zakia ; Xu, Cynthia ; Xie, Xuan ; Delgado-Fukushima, Erika ; Wadghiri, Youssef Z. ; Montclare, Jin. / Protein-engineered nanoscale micelles for dynamic 19 F magnetic resonance and therapeutic drug delivery In: ACS Nano. 2019.
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