Protein aggregation measurement through electrical impedance spectroscopy

A. Affanni, A. Corazza, Gennaro Esposito, F. Fogolari, M. Polano

Research output: Contribution to journalConference article

Abstract

The paper presents a novel methodology to measure the fibril formation in protein solutions. We designed a bench consisting of a sensor having interdigitated electrodes, a PDMS hermetic reservoir and an impedance meter automatically driven by calculator. The impedance data are interpolated with a lumped elements model and their change over time can provide information on the aggregation process. Encouraging results have been obtained by testing the methodology on K-casein, a protein of milk, with and without the addition of a drug inhibiting the aggregation. The amount of sample needed to perform this measurement is by far lower than the amount needed by fluorescence analysis.

Original languageEnglish (US)
Article number012049
JournalJournal of Physics: Conference Series
Volume459
Issue number1
DOIs
StatePublished - Jan 1 2013
Event2013 Joint IMEKO (International Measurement Confederation) TC1-TC7-TC13 Symposium: Measurement Across Physical and Behavioural Sciences - Genoa, Italy
Duration: Sep 4 2013Sep 6 2013

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electrical impedance
impedance
methodology
proteins
calculators
milk
spectroscopy
seats
drugs
fluorescence
electrodes
sensors

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Protein aggregation measurement through electrical impedance spectroscopy. / Affanni, A.; Corazza, A.; Esposito, Gennaro; Fogolari, F.; Polano, M.

In: Journal of Physics: Conference Series, Vol. 459, No. 1, 012049, 01.01.2013.

Research output: Contribution to journalConference article

Affanni, A. ; Corazza, A. ; Esposito, Gennaro ; Fogolari, F. ; Polano, M. / Protein aggregation measurement through electrical impedance spectroscopy. In: Journal of Physics: Conference Series. 2013 ; Vol. 459, No. 1.
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