Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome

Nestor N. Jimenez-Vargas, Luke A. Pattison, Peishen Zhao, Tina Marie Lieu, Rocco Latorre, Dane D. Jensen, Joel Castro, Luigi Aurelio, Giang T. Le, Bernard Flynn, Carmen Klein Herenbrink, Holly R. Yeatman, Laura Edgington-Mitchell, Christopher J.H. Porter, Michelle L. Halls, Meritxell Canals, Nicholas A. Veldhuis, Daniel P. Poole, Peter McLean, Gareth A. HicksNicole Scheff, Elyssa Chen, Aditi Bhattacharya, Brian Schmidt, Stuart M. Brierley, Stephen J. Vanner, Nigel W. Bunnett

Research output: Contribution to journalArticle

Abstract

Once activated at the surface of cells, G protein-coupled receptors (GPCRs) redistribute to endosomes, where they can continue to signal. Whether GPCRs in endosomes generate signals that contribute to human disease is unknown. We evaluated endosomal signaling of protease-activated receptor-2 (PAR2), which has been proposed to mediate pain in patients with irritable bowel syndrome (IBS). Trypsin, elastase, and cathepsin S, which are activated in the colonic mucosa of patients with IBS and in experimental animals with colitis, caused persistent PAR2-dependent hyperexcitability of nociceptors, sensitization of colonic afferent neurons to mechanical stimuli, and somatic mechanical allodynia. Inhibitors of clathrin- and dynamin-dependent endocytosis and of mitogen-activated protein kinase kinase-1 prevented trypsin-induced hyperexcitability, sensitization, and allodynia. However, they did not affect elastase- or cathepsin S-induced hyperexcitability, sensitization, or allodynia. Trypsin stimulated endocytosis of PAR2, which signaled from endosomes to activate extracellular signal-regulated kinase. Elastase and cathepsin S did not stimulate endocytosis of PAR2, which signaled from the plasma membrane to activate adenylyl cyclase. Biopsies of colonic mucosa from IBS patients released proteases that induced persistent PAR2-dependent hyperexcitability of nociceptors, and PAR2 association with β-arrestins, which mediate endocytosis. Conjugation to cholestanol promoted delivery and retention of antagonists in endosomes containing PAR2. A cholestanol-conjugated PAR2 antagonist prevented persistent trypsin- and IBS protease-induced hyperexcitability of nociceptors. The results reveal that PAR2 signaling from endosomes underlies the persistent hyperexcitability of nociceptors that mediates chronic pain of IBS. Endosomally targeted PAR2 antagonists are potential therapies for IBS pain. GPCRs in endosomes transmit signals that contribute to human diseases.

Original languageEnglish (US)
Pages (from-to)E7438-E7447
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number31
DOIs
StatePublished - Jul 31 2018

Fingerprint

PAR-2 Receptor
Irritable Bowel Syndrome
Endosomes
cathepsin S
Pain
Nociceptors
Endocytosis
Trypsin
Pancreatic Elastase
Hyperalgesia
G-Protein-Coupled Receptors
Cholestanol
Mucous Membrane
Peptide Hydrolases
Arrestins
MAP Kinase Kinase 1
Dynamins
Afferent Neurons
Clathrin
Extracellular Signal-Regulated MAP Kinases

Keywords

  • Endosomes
  • Pain
  • Proteases
  • Receptors

ASJC Scopus subject areas

  • General

Cite this

Jimenez-Vargas, N. N., Pattison, L. A., Zhao, P., Lieu, T. M., Latorre, R., Jensen, D. D., ... Bunnett, N. W. (2018). Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome. Proceedings of the National Academy of Sciences of the United States of America, 115(31), E7438-E7447. https://doi.org/10.1073/pnas.1721891115

Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome. / Jimenez-Vargas, Nestor N.; Pattison, Luke A.; Zhao, Peishen; Lieu, Tina Marie; Latorre, Rocco; Jensen, Dane D.; Castro, Joel; Aurelio, Luigi; Le, Giang T.; Flynn, Bernard; Herenbrink, Carmen Klein; Yeatman, Holly R.; Edgington-Mitchell, Laura; Porter, Christopher J.H.; Halls, Michelle L.; Canals, Meritxell; Veldhuis, Nicholas A.; Poole, Daniel P.; McLean, Peter; Hicks, Gareth A.; Scheff, Nicole; Chen, Elyssa; Bhattacharya, Aditi; Schmidt, Brian; Brierley, Stuart M.; Vanner, Stephen J.; Bunnett, Nigel W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 31, 31.07.2018, p. E7438-E7447.

Research output: Contribution to journalArticle

Jimenez-Vargas, NN, Pattison, LA, Zhao, P, Lieu, TM, Latorre, R, Jensen, DD, Castro, J, Aurelio, L, Le, GT, Flynn, B, Herenbrink, CK, Yeatman, HR, Edgington-Mitchell, L, Porter, CJH, Halls, ML, Canals, M, Veldhuis, NA, Poole, DP, McLean, P, Hicks, GA, Scheff, N, Chen, E, Bhattacharya, A, Schmidt, B, Brierley, SM, Vanner, SJ & Bunnett, NW 2018, 'Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 31, pp. E7438-E7447. https://doi.org/10.1073/pnas.1721891115
Jimenez-Vargas, Nestor N. ; Pattison, Luke A. ; Zhao, Peishen ; Lieu, Tina Marie ; Latorre, Rocco ; Jensen, Dane D. ; Castro, Joel ; Aurelio, Luigi ; Le, Giang T. ; Flynn, Bernard ; Herenbrink, Carmen Klein ; Yeatman, Holly R. ; Edgington-Mitchell, Laura ; Porter, Christopher J.H. ; Halls, Michelle L. ; Canals, Meritxell ; Veldhuis, Nicholas A. ; Poole, Daniel P. ; McLean, Peter ; Hicks, Gareth A. ; Scheff, Nicole ; Chen, Elyssa ; Bhattacharya, Aditi ; Schmidt, Brian ; Brierley, Stuart M. ; Vanner, Stephen J. ; Bunnett, Nigel W. / Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 31. pp. E7438-E7447.
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AU - Jimenez-Vargas, Nestor N.

AU - Pattison, Luke A.

AU - Zhao, Peishen

AU - Lieu, Tina Marie

AU - Latorre, Rocco

AU - Jensen, Dane D.

AU - Castro, Joel

AU - Aurelio, Luigi

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AU - Flynn, Bernard

AU - Herenbrink, Carmen Klein

AU - Yeatman, Holly R.

AU - Edgington-Mitchell, Laura

AU - Porter, Christopher J.H.

AU - Halls, Michelle L.

AU - Canals, Meritxell

AU - Veldhuis, Nicholas A.

AU - Poole, Daniel P.

AU - McLean, Peter

AU - Hicks, Gareth A.

AU - Scheff, Nicole

AU - Chen, Elyssa

AU - Bhattacharya, Aditi

AU - Schmidt, Brian

AU - Brierley, Stuart M.

AU - Vanner, Stephen J.

AU - Bunnett, Nigel W.

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