Prostanoids induce egr1 gene expression in cementoblastic OCCM cells

L. Pham, O. Bezouglaia, P. M. Camargo, Jeanne Nervina, S. Tetradis

Research output: Contribution to journalArticle

Abstract

Background and Objective: Prostanoids that activate protein kinase C signaling are potent anabolic stimulators of cementoblastic OCCM cells. Using cDNA subtractive hybridization, we identified early growth response gene-1 (egr1) as a prostanoid-induced gene. Egr1, a zinc-finger transcription factor expressed during tooth development, regulates cell growth and differentiation. We hypothesize that Egr1 may mediate part of the prostanoid-induced anabolic effect in cementoblasts. Our objective was to characterize prostanoid-induced egr1 gene expression in OCCM cells. Material and Methods: Total RNA and proteins were assayed by northern blot and western immunoblot assays. Results: Prostaglandin E2-, prostaglandin F- and fluprostenol-induced egr1 mRNA levels peaked at 0.5 h and returned to baseline by 4 h. Prostaglandin F2α and fluprostenol more potently induced egr1 compared with prostaglandin E2. The phorbol ester, phorbol 12-myristate 13-acetate, which activates protein kinase C signaling, induced egr1 mRNA levels 66-fold over the control, whereas forskolin (a cAMP-protein kinase A activator) and ionomycin (a calcium activator) had no effect. Protein kinase C inhibition significantly inhibited prostaglandin E 2-, prostaglandin F- and fluprostenol-induced egr1 mRNA levels. Finally, prostanoids maximally induced Egr1 protein at 1 h. Conclusion: egr1 is a primary response gene induced by prostaglandin E 2, prostaglandin F and fluprostenol in OCCM cells through protein kinase C signaling, suggesting that Egr1 may be a key mediator of anabolic responses in cementoblasts. Cementum is vital for periodontal organ maintenance and regeneration. Periodontal ligament fibers (Sharpey's fibers) insert into bone and cementum, thereby supporting the tooth in the alveolus (1). If the periodontal organ is lost, its regeneration requires cementoblast differentiation in order to form new cementum for periodontal ligament fiber insertion. Early attempts to regenerate cementum have proven difficult and rarely generate sufficient tissue (2). A better understanding of the molecular and cellular regulators that promote cementoblast differentiation is critical for developing targeted periodontal regeneration.

Original languageEnglish (US)
Pages (from-to)486-493
Number of pages8
JournalJournal of Periodontal Research
Volume42
Issue number5
DOIs
StatePublished - Oct 2007

Fingerprint

Dental Cementum
Prostaglandins
Gene Expression
Dinoprost
Growth
Genes
Protein Kinase C
Regeneration
Periodontal Ligament
Prostaglandins E
Dinoprostone
Messenger RNA
Tooth
Anabolic Agents
Ionomycin
Zinc Fingers
Phorbol Esters
Colforsin
Cyclic AMP-Dependent Protein Kinases
Northern Blotting

Keywords

  • Cementoblasts
  • egr1
  • Prostanoids
  • Signaling

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Pham, L., Bezouglaia, O., Camargo, P. M., Nervina, J., & Tetradis, S. (2007). Prostanoids induce egr1 gene expression in cementoblastic OCCM cells. Journal of Periodontal Research, 42(5), 486-493. https://doi.org/10.1111/j.1600-0765.2007.00972.x

Prostanoids induce egr1 gene expression in cementoblastic OCCM cells. / Pham, L.; Bezouglaia, O.; Camargo, P. M.; Nervina, Jeanne; Tetradis, S.

In: Journal of Periodontal Research, Vol. 42, No. 5, 10.2007, p. 486-493.

Research output: Contribution to journalArticle

Pham, L, Bezouglaia, O, Camargo, PM, Nervina, J & Tetradis, S 2007, 'Prostanoids induce egr1 gene expression in cementoblastic OCCM cells', Journal of Periodontal Research, vol. 42, no. 5, pp. 486-493. https://doi.org/10.1111/j.1600-0765.2007.00972.x
Pham, L. ; Bezouglaia, O. ; Camargo, P. M. ; Nervina, Jeanne ; Tetradis, S. / Prostanoids induce egr1 gene expression in cementoblastic OCCM cells. In: Journal of Periodontal Research. 2007 ; Vol. 42, No. 5. pp. 486-493.
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abstract = "Background and Objective: Prostanoids that activate protein kinase C signaling are potent anabolic stimulators of cementoblastic OCCM cells. Using cDNA subtractive hybridization, we identified early growth response gene-1 (egr1) as a prostanoid-induced gene. Egr1, a zinc-finger transcription factor expressed during tooth development, regulates cell growth and differentiation. We hypothesize that Egr1 may mediate part of the prostanoid-induced anabolic effect in cementoblasts. Our objective was to characterize prostanoid-induced egr1 gene expression in OCCM cells. Material and Methods: Total RNA and proteins were assayed by northern blot and western immunoblot assays. Results: Prostaglandin E2-, prostaglandin F2α- and fluprostenol-induced egr1 mRNA levels peaked at 0.5 h and returned to baseline by 4 h. Prostaglandin F2α and fluprostenol more potently induced egr1 compared with prostaglandin E2. The phorbol ester, phorbol 12-myristate 13-acetate, which activates protein kinase C signaling, induced egr1 mRNA levels 66-fold over the control, whereas forskolin (a cAMP-protein kinase A activator) and ionomycin (a calcium activator) had no effect. Protein kinase C inhibition significantly inhibited prostaglandin E 2-, prostaglandin F2α- and fluprostenol-induced egr1 mRNA levels. Finally, prostanoids maximally induced Egr1 protein at 1 h. Conclusion: egr1 is a primary response gene induced by prostaglandin E 2, prostaglandin F2α and fluprostenol in OCCM cells through protein kinase C signaling, suggesting that Egr1 may be a key mediator of anabolic responses in cementoblasts. Cementum is vital for periodontal organ maintenance and regeneration. Periodontal ligament fibers (Sharpey's fibers) insert into bone and cementum, thereby supporting the tooth in the alveolus (1). If the periodontal organ is lost, its regeneration requires cementoblast differentiation in order to form new cementum for periodontal ligament fiber insertion. Early attempts to regenerate cementum have proven difficult and rarely generate sufficient tissue (2). A better understanding of the molecular and cellular regulators that promote cementoblast differentiation is critical for developing targeted periodontal regeneration.",
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