Prostaglandin E2 stimulates bone sialoprotein (BSP) expression through cAMP and fibroblast growth factor 2 response elements in the proximal promoter of the rat BSP gene

Hiroshi Samoto, Emi Shimizu, Yuko Matsuda-Honjyo, Ryoichiro Saito, Sumi Nakao, Muneyoshi Yamazaki, Shunsuke Furuyama, Hiroshi Sugiya, Jaro Sodek, Yorimasa Ogata

Research output: Contribution to journalArticle

Abstract

Bone sialoprotein (BSP), an early marker of osteoblast differentiation, has been implicated in the nucleation of hydroxyapatite during de novo bone formation. Prostaglandin E2 (PGE2) has anabolic effects on proliferation and differentiation of osteoblasts via diverse signal transduction systems. Because PGE2 increases the proportion of functional osteoblasts in fetal rat calvarial cell cultures, we investigated the regulation of BSP, as an osteoblastic marker, by PGE2. Treatment of rat osteosarcoma UMR 106 cells with 3 μM, 300 μM, and 30 nM PGE2 increased the steady state levels of BSP mRNA about 2.7-, 2.5-, and 2.4-fold after 12 h. From transient transfection assays, the constructs including the promoter sequence of nucleotides (nt) - 116 to +60 (pLUC3) were found to enhance transcriptional activity 3.8- and 2.2-fold treated with 3 μM and 30 μM PGE2 for 12 h. 2-bp mutations were made in an inverted CCAAT box (between nt -50 and -46), a cAMP response element (CRE; between nt -75 and -68), a fibroblast growth factor 2 response element (FRE; nt -92 to -85), and a pituitary-specific transcription factor-1 motif (between nt -111 and -105) within pLUC3 and pLUC7 constructs. Transcriptional stimulation by PGE2 was almost completed abrogated in constructs that included 2-bp mutations in either the CRE and FRE. In gel shift analyses an increased binding of nuclear extract components to double-tranded oligonucleotide probes containing CRE and FRE was observed following treatment with PGE2. These studies show that PGE2 induces BSP transcription in UMR 106 cells through juxtaposed CRE and FRE elements in the proximal promoter of the BSP gene.

Original languageEnglish (US)
Pages (from-to)28659-28667
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number31
DOIs
StatePublished - Aug 1 2003

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Integrin-Binding Sialoprotein
Response Elements
Fibroblast Growth Factor 2
Dinoprostone
Rats
Genes
Nucleotides
Osteoblasts
Anabolic Agents
Signal transduction
Mutation
Nucleotide Motifs
Oligonucleotide Probes
rat Spp1 protein
Differentiation Antigens
Electrophoretic Mobility Shift Assay
Osteosarcoma
Transcription
Durapatite
Cell culture

ASJC Scopus subject areas

  • Biochemistry

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Prostaglandin E2 stimulates bone sialoprotein (BSP) expression through cAMP and fibroblast growth factor 2 response elements in the proximal promoter of the rat BSP gene. / Samoto, Hiroshi; Shimizu, Emi; Matsuda-Honjyo, Yuko; Saito, Ryoichiro; Nakao, Sumi; Yamazaki, Muneyoshi; Furuyama, Shunsuke; Sugiya, Hiroshi; Sodek, Jaro; Ogata, Yorimasa.

In: Journal of Biological Chemistry, Vol. 278, No. 31, 01.08.2003, p. 28659-28667.

Research output: Contribution to journalArticle

Samoto, H, Shimizu, E, Matsuda-Honjyo, Y, Saito, R, Nakao, S, Yamazaki, M, Furuyama, S, Sugiya, H, Sodek, J & Ogata, Y 2003, 'Prostaglandin E2 stimulates bone sialoprotein (BSP) expression through cAMP and fibroblast growth factor 2 response elements in the proximal promoter of the rat BSP gene', Journal of Biological Chemistry, vol. 278, no. 31, pp. 28659-28667. https://doi.org/10.1074/jbc.M300671200
Samoto, Hiroshi ; Shimizu, Emi ; Matsuda-Honjyo, Yuko ; Saito, Ryoichiro ; Nakao, Sumi ; Yamazaki, Muneyoshi ; Furuyama, Shunsuke ; Sugiya, Hiroshi ; Sodek, Jaro ; Ogata, Yorimasa. / Prostaglandin E2 stimulates bone sialoprotein (BSP) expression through cAMP and fibroblast growth factor 2 response elements in the proximal promoter of the rat BSP gene. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 31. pp. 28659-28667.
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AU - Shimizu, Emi

AU - Matsuda-Honjyo, Yuko

AU - Saito, Ryoichiro

AU - Nakao, Sumi

AU - Yamazaki, Muneyoshi

AU - Furuyama, Shunsuke

AU - Sugiya, Hiroshi

AU - Sodek, Jaro

AU - Ogata, Yorimasa

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