Propagation of temporal and rate signals in cultured multilayer networks

Jérémie Barral, Xiao Jing Wang, Alex D. Reyes

Research output: Contribution to journalArticle

Abstract

Analyses of idealized feedforward networks suggest that several conditions have to be satisfied in order for activity to propagate faithfully across layers. Verifying these concepts experimentally has been difficult owing to the vast number of variables that must be controlled. Here, we cultured cortical neurons in a chamber with sequentially connected compartments, optogenetically stimulated individual neurons in the first layer with high spatiotemporal resolution, and then monitored the subthreshold and suprathreshold potentials in subsequent layers. Brief stimuli delivered to the first layer evoked a short-latency transient response followed by sustained activity. Rate signals, carried by the sustained component, propagated reliably through 4 layers, unlike idealized feedforward networks, which tended strongly towards synchrony. Moreover, temporal jitter in the stimulus was transformed into a rate code and transmitted to the last layer. This novel mode of propagation occurred in the balanced excitatory-inhibitory regime and is mediated by NMDA-mediated receptors and recurrent activity.

Original languageEnglish (US)
Article number3969
JournalNature Communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

Neurons
Multilayers
propagation
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Jitter
Transient analysis
Reaction Time
neurons
stimuli
transient response
compartments
chambers
vibration
high resolution

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Propagation of temporal and rate signals in cultured multilayer networks. / Barral, Jérémie; Wang, Xiao Jing; Reyes, Alex D.

In: Nature Communications, Vol. 10, No. 1, 3969, 01.12.2019.

Research output: Contribution to journalArticle

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