Processing, characterization, and in vitro/in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr alloys

Marco C. Bottino, Paulo Coelho, Vinicius A R Henriques, Olga Z. Higa, Ana H A Bressiani, José C. Bressiani

Research output: Contribution to journalArticle

Abstract

This article presents details of processing, characterization and in vitro as well as in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr samples with different levels of porosity. Sintered samples were characterized for density, crystalline phases (XRD), and micro-structure (SEM and EDX). Samples sintered at 1000°C showed the highest porosity level (∼30%), featuring open and interconnected pores ranging from 50 to 100 uμm in diameter but incomplete densification. In contrast, samples sintered at 1300 and 1500°C demonstrated high densification with 10% porosity level distributed in a homogeneous microstructure. The different sintering conditions used in this study demonstrated a coherent trend that is increase in temperature lead to higher sample densification, even though densification represents a drawback for bone ingrowth. Cytotoxicity tests did not reveal any toxic effects of the starting and processed materials on surviving cell percentage. After an 8-week healing period in rabbit tibias, the implants were retrieved, processed for nondecalcified histological evaluation, and then assessed by backscattered electron images (BSEI-SEM) and EDX. Bone growth into the microstructure was observed only in samples sintered at 1000°C. Overall, a close relation between newly formed bone and all processed samples was observed.

Original languageEnglish (US)
Pages (from-to)689-696
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume88
Issue number3
DOIs
StatePublished - Mar 1 2009

Fingerprint

Powder metallurgy
Densification
Bone
Porosity
Processing
Microstructure
Energy dispersive spectroscopy
Scanning electron microscopy
Poisons
Phase structure
Cytotoxicity
Density (specific gravity)
Sintering
Crystalline materials
titanium alloy (TiNb13Zr13)
Electrons
Temperature

Keywords

  • Cytotoxicity
  • Osseointegration
  • Powder metallurgy
  • Sintering
  • Titanium alloys

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Processing, characterization, and in vitro/in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr alloys. / Bottino, Marco C.; Coelho, Paulo; Henriques, Vinicius A R; Higa, Olga Z.; Bressiani, Ana H A; Bressiani, José C.

In: Journal of Biomedical Materials Research - Part A, Vol. 88, No. 3, 01.03.2009, p. 689-696.

Research output: Contribution to journalArticle

Bottino, Marco C. ; Coelho, Paulo ; Henriques, Vinicius A R ; Higa, Olga Z. ; Bressiani, Ana H A ; Bressiani, José C. / Processing, characterization, and in vitro/in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr alloys. In: Journal of Biomedical Materials Research - Part A. 2009 ; Vol. 88, No. 3. pp. 689-696.
@article{a01a97dc0e9545149333cd46e48d2140,
title = "Processing, characterization, and in vitro/in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr alloys",
abstract = "This article presents details of processing, characterization and in vitro as well as in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr samples with different levels of porosity. Sintered samples were characterized for density, crystalline phases (XRD), and micro-structure (SEM and EDX). Samples sintered at 1000°C showed the highest porosity level (∼30{\%}), featuring open and interconnected pores ranging from 50 to 100 uμm in diameter but incomplete densification. In contrast, samples sintered at 1300 and 1500°C demonstrated high densification with 10{\%} porosity level distributed in a homogeneous microstructure. The different sintering conditions used in this study demonstrated a coherent trend that is increase in temperature lead to higher sample densification, even though densification represents a drawback for bone ingrowth. Cytotoxicity tests did not reveal any toxic effects of the starting and processed materials on surviving cell percentage. After an 8-week healing period in rabbit tibias, the implants were retrieved, processed for nondecalcified histological evaluation, and then assessed by backscattered electron images (BSEI-SEM) and EDX. Bone growth into the microstructure was observed only in samples sintered at 1000°C. Overall, a close relation between newly formed bone and all processed samples was observed.",
keywords = "Cytotoxicity, Osseointegration, Powder metallurgy, Sintering, Titanium alloys",
author = "Bottino, {Marco C.} and Paulo Coelho and Henriques, {Vinicius A R} and Higa, {Olga Z.} and Bressiani, {Ana H A} and Bressiani, {Jos{\'e} C.}",
year = "2009",
month = "3",
day = "1",
doi = "10.1002/jbm.a.31912",
language = "English (US)",
volume = "88",
pages = "689--696",
journal = "Journal of Biomedical Materials Research",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Processing, characterization, and in vitro/in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr alloys

AU - Bottino, Marco C.

AU - Coelho, Paulo

AU - Henriques, Vinicius A R

AU - Higa, Olga Z.

AU - Bressiani, Ana H A

AU - Bressiani, José C.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - This article presents details of processing, characterization and in vitro as well as in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr samples with different levels of porosity. Sintered samples were characterized for density, crystalline phases (XRD), and micro-structure (SEM and EDX). Samples sintered at 1000°C showed the highest porosity level (∼30%), featuring open and interconnected pores ranging from 50 to 100 uμm in diameter but incomplete densification. In contrast, samples sintered at 1300 and 1500°C demonstrated high densification with 10% porosity level distributed in a homogeneous microstructure. The different sintering conditions used in this study demonstrated a coherent trend that is increase in temperature lead to higher sample densification, even though densification represents a drawback for bone ingrowth. Cytotoxicity tests did not reveal any toxic effects of the starting and processed materials on surviving cell percentage. After an 8-week healing period in rabbit tibias, the implants were retrieved, processed for nondecalcified histological evaluation, and then assessed by backscattered electron images (BSEI-SEM) and EDX. Bone growth into the microstructure was observed only in samples sintered at 1000°C. Overall, a close relation between newly formed bone and all processed samples was observed.

AB - This article presents details of processing, characterization and in vitro as well as in vivo evaluations of powder metallurgy processed Ti-13Nb-13Zr samples with different levels of porosity. Sintered samples were characterized for density, crystalline phases (XRD), and micro-structure (SEM and EDX). Samples sintered at 1000°C showed the highest porosity level (∼30%), featuring open and interconnected pores ranging from 50 to 100 uμm in diameter but incomplete densification. In contrast, samples sintered at 1300 and 1500°C demonstrated high densification with 10% porosity level distributed in a homogeneous microstructure. The different sintering conditions used in this study demonstrated a coherent trend that is increase in temperature lead to higher sample densification, even though densification represents a drawback for bone ingrowth. Cytotoxicity tests did not reveal any toxic effects of the starting and processed materials on surviving cell percentage. After an 8-week healing period in rabbit tibias, the implants were retrieved, processed for nondecalcified histological evaluation, and then assessed by backscattered electron images (BSEI-SEM) and EDX. Bone growth into the microstructure was observed only in samples sintered at 1000°C. Overall, a close relation between newly formed bone and all processed samples was observed.

KW - Cytotoxicity

KW - Osseointegration

KW - Powder metallurgy

KW - Sintering

KW - Titanium alloys

UR - http://www.scopus.com/inward/record.url?scp=60549114723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549114723&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.31912

DO - 10.1002/jbm.a.31912

M3 - Article

VL - 88

SP - 689

EP - 696

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 1549-3296

IS - 3

ER -