Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas

Hong Qiao, Zhouxin Shen, Shao-Shan Huang, Robert J. Schmitz, Mark A. Urich, Steven P. Briggs, Joseph R. Ecker

Research output: Contribution to journalArticle

Abstract

Ethylene gas is essential for many developmental processes and stress responses in plants. ETHYLENE INSENSITIVE2 (EIN2), an NRAMP-like integral membrane protein, plays an essential role in ethylene signaling, but its function remains enigmatic. Here we report that phosphorylation-regulated proteolytic processing of EIN2 triggers its endoplasmic reticulum (ER) - to - nucleus translocation. ER-tethered EIN2 shows CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) kinase - dependent phosphorylation. Ethylene triggers dephosphorylation at several sites and proteolytic cleavage at one of these sites, resulting in nuclear translocation of a carboxyl-terminal EIN2 fragment (EIN2-C′). Mutations that mimic EIN2 dephosphorylation, or inactivate CTR1, show constitutive cleavage and nuclear localization of EIN2-C′ and EIN3 and EIN3-LIKE1 - dependent activation of ethylene responses. These findings uncover a mechanism of subcellular communication whereby ethylene stimulates phosphorylation-dependent cleavage and nuclear movement of the EIN2-C′ peptide, linking hormone perception and signaling components in the ER with nuclear-localized transcriptional regulators.

Original languageEnglish (US)
Pages (from-to)390-393
Number of pages4
JournalScience
Volume338
Issue number6105
DOIs
StatePublished - Oct 19 2012

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Endoplasmic Reticulum
Gases
Phosphorylation
ethylene
Peptide Hormones
C-Peptide
Membrane Proteins
Phosphotransferases
Communication

ASJC Scopus subject areas

  • General

Cite this

Qiao, H., Shen, Z., Huang, S-S., Schmitz, R. J., Urich, M. A., Briggs, S. P., & Ecker, J. R. (2012). Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. Science, 338(6105), 390-393. https://doi.org/10.1126/science.1225974

Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. / Qiao, Hong; Shen, Zhouxin; Huang, Shao-Shan; Schmitz, Robert J.; Urich, Mark A.; Briggs, Steven P.; Ecker, Joseph R.

In: Science, Vol. 338, No. 6105, 19.10.2012, p. 390-393.

Research output: Contribution to journalArticle

Qiao, H, Shen, Z, Huang, S-S, Schmitz, RJ, Urich, MA, Briggs, SP & Ecker, JR 2012, 'Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas', Science, vol. 338, no. 6105, pp. 390-393. https://doi.org/10.1126/science.1225974
Qiao H, Shen Z, Huang S-S, Schmitz RJ, Urich MA, Briggs SP et al. Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. Science. 2012 Oct 19;338(6105):390-393. https://doi.org/10.1126/science.1225974
Qiao, Hong ; Shen, Zhouxin ; Huang, Shao-Shan ; Schmitz, Robert J. ; Urich, Mark A. ; Briggs, Steven P. ; Ecker, Joseph R. / Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. In: Science. 2012 ; Vol. 338, No. 6105. pp. 390-393.
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