Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein

Giorgia Brancolini, Alessandra Corazza, Marco Vuano, Federico Fogolari, Maria Chiara Mimmi, Vittorio Bellotti, Monica Stoppini, Stefano Corni, Gennaro Esposito

Research output: Contribution to journalArticle

Abstract

Nanoparticles (NPs) are known to exhibit distinct physical and chemical properties compared with the same materials in bulk form. NPs have been repeatedly reported to interact with proteins, and this interaction can be exploited to affect processes undergone by proteins, such as fibrillogenesis. Fibrillation is common to many proteins, and in living organisms, it causes tissue-specific or systemic amyloid diseases. The nature of NPs and their surface chemistry is crucial in assessing their affinity for proteins and their effects on them. Here we present the first detailed structural characterization and molecular mechanics model of the interaction between a fibrillogenic protein, β2-microglobulin, and a NP, 5 nm hydrophilic citrate-capped gold nanoparticles. NMR measurements and simulations at multiple levels (enhanced sampling molecular dynamics, Brownian dynamics, and Poisson-Boltzmann electrostatics) explain the origin of the observed protein perturbations mostly localized at the amino-terminal region. Experiments show that the protein-NP interaction is weak in the physiological-like, conditions and do not induce protein fibrillation. Simulations reproduce these findings and reveal instead the role of the citrate in destabilizing the lower pH protonated form of β2-microglobulin. The results offer possible strategies for controlling the desired effect of NPs on the conformational changes of the proteins, which have significant roles in the fibrillation process.

Original languageEnglish (US)
Pages (from-to)2600-2613
Number of pages14
JournalACS Nano
Volume9
Issue number3
DOIs
StatePublished - Jan 1 2015

Fingerprint

Amyloidogenic Proteins
citrates
Citric Acid
Gold
gold
Nanoparticles
proteins
Proteins
nanoparticles
fibrillation
Molecular mechanics
Surface chemistry
organisms
Amyloid
chemical properties
Chemical properties
affinity
Molecular dynamics
Electrostatics
simulation

Keywords

  • amyloid
  • docking
  • fibrillogenesis
  • molecular dynamics
  • nanoparticles
  • nuclear magnetic resonance

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Brancolini, G., Corazza, A., Vuano, M., Fogolari, F., Mimmi, M. C., Bellotti, V., ... Esposito, G. (2015). Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein. ACS Nano, 9(3), 2600-2613. https://doi.org/10.1021/nn506161j

Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein. / Brancolini, Giorgia; Corazza, Alessandra; Vuano, Marco; Fogolari, Federico; Mimmi, Maria Chiara; Bellotti, Vittorio; Stoppini, Monica; Corni, Stefano; Esposito, Gennaro.

In: ACS Nano, Vol. 9, No. 3, 01.01.2015, p. 2600-2613.

Research output: Contribution to journalArticle

Brancolini, G, Corazza, A, Vuano, M, Fogolari, F, Mimmi, MC, Bellotti, V, Stoppini, M, Corni, S & Esposito, G 2015, 'Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein', ACS Nano, vol. 9, no. 3, pp. 2600-2613. https://doi.org/10.1021/nn506161j
Brancolini G, Corazza A, Vuano M, Fogolari F, Mimmi MC, Bellotti V et al. Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein. ACS Nano. 2015 Jan 1;9(3):2600-2613. https://doi.org/10.1021/nn506161j
Brancolini, Giorgia ; Corazza, Alessandra ; Vuano, Marco ; Fogolari, Federico ; Mimmi, Maria Chiara ; Bellotti, Vittorio ; Stoppini, Monica ; Corni, Stefano ; Esposito, Gennaro. / Probing the influence of citrate-capped gold nanoparticles on an amyloidogenic protein. In: ACS Nano. 2015 ; Vol. 9, No. 3. pp. 2600-2613.
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