Priming and induction of anti-rotavirus antibody response by synthetic peptides derived from VP7 and VP4

M. K. Ijaz, T. O. Alkarmi, A. W. El-Mekki, Sehamuddin Galadari, F. K. Dar, L. A. Babiuk

    Research output: Contribution to journalArticle

    Abstract

    Synthetic peptides derived from bovine rotavirus C-486 (BRV) outer capsid (VP7 and VP4) and inner capsid (VP6) proteins were tested to evaluate their ability to prime and induce an anti-rotavirus antibody response. Peptides corresponding to the amino acid residues 232-255 of VP4 (VP4-peptide), 275-295 of VP7 (VP7-peptide) and 40-60 of VP6 (VP6-peptide) of BRV were chemically synthesized. These peptides were coupled to carrier proteins (either keyhole limpet haemocyanin (KLH) or recombinant rotavirus inner capsid protein-VP6 assembled into virus-like particles (VP6-carrier) were used as carrier to link the synthetic peptides under study), and the resulting conjugates were used to immunize rotavirus seronegative mice. An enzyme-linked immunosorbent assay (ELISA) was used to determine anti-peptide and anti-rotavirus antibody titres in serum samples collected after immunization. All peptides were immunogenic in mice and induced the production of anti-peptide antibodies, but with the exception of VP6-peptide they were not able to induce anti-rotavirus antibodies as measured by ELISA. Western blot analysis indicated that antibodies against each peptide were able to react with the respective authentic viral proteins of various rotavirus serotypes. To determine if a peptide-primed animal would respond to native viral proteins, animals were subsequently injected with purified BRV. A rapid and high anti-rotavirus antibody titre, in addition to a rise in anti-peptide antibody titre, was observed in peptide-primed mice. Furthermore, the sera obtained from these mice neutralized the virus under in vitro conditions. The significance of these results in relation to a potential rotavirus synthetic peptide-based vaccine is discussed.

    Original languageEnglish (US)
    Pages (from-to)331-338
    Number of pages8
    JournalVaccine
    Volume13
    Issue number4
    DOIs
    StatePublished - Jan 1 1995

    Fingerprint

    synthetic peptides
    Rotavirus
    Antibody Formation
    Anti-Idiotypic Antibodies
    peptides
    Peptides
    antibodies
    Rotavirus C
    viral proteins
    mice
    coat proteins
    Capsid Proteins
    Viral Proteins
    cattle
    enzyme-linked immunosorbent assay
    Enzyme-Linked Immunosorbent Assay
    virus-like particles
    capsid
    transport proteins
    Synthetic Vaccines

    Keywords

    • antibody response
    • Rotavirus
    • synthetic peptides

    ASJC Scopus subject areas

    • Molecular Medicine
    • Immunology and Microbiology(all)
    • veterinary(all)
    • Public Health, Environmental and Occupational Health
    • Infectious Diseases

    Cite this

    Ijaz, M. K., Alkarmi, T. O., El-Mekki, A. W., Galadari, S., Dar, F. K., & Babiuk, L. A. (1995). Priming and induction of anti-rotavirus antibody response by synthetic peptides derived from VP7 and VP4. Vaccine, 13(4), 331-338. https://doi.org/10.1016/0264-410X(95)98252-6

    Priming and induction of anti-rotavirus antibody response by synthetic peptides derived from VP7 and VP4. / Ijaz, M. K.; Alkarmi, T. O.; El-Mekki, A. W.; Galadari, Sehamuddin; Dar, F. K.; Babiuk, L. A.

    In: Vaccine, Vol. 13, No. 4, 01.01.1995, p. 331-338.

    Research output: Contribution to journalArticle

    Ijaz, MK, Alkarmi, TO, El-Mekki, AW, Galadari, S, Dar, FK & Babiuk, LA 1995, 'Priming and induction of anti-rotavirus antibody response by synthetic peptides derived from VP7 and VP4', Vaccine, vol. 13, no. 4, pp. 331-338. https://doi.org/10.1016/0264-410X(95)98252-6
    Ijaz, M. K. ; Alkarmi, T. O. ; El-Mekki, A. W. ; Galadari, Sehamuddin ; Dar, F. K. ; Babiuk, L. A. / Priming and induction of anti-rotavirus antibody response by synthetic peptides derived from VP7 and VP4. In: Vaccine. 1995 ; Vol. 13, No. 4. pp. 331-338.
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