Primary culture of canine prostate epithelial cells

Louis Terracio, William H J Douglas

Research output: Contribution to journalArticle

Abstract

Epithelial-cell enriched primary cultures were established from canine prostate by a collagenase digestion and selective attachment procedure. The epithelial cells in primary culture retained differentiated structure and function. The epithelial cells were attached to one another by tight junctions and desmosomes to form "lumenlike structures" that resemble the acini of the intact organ and appeared to contain typical protein synthetic organelles (1,2). The cultures contained significant levels of acid phosphatase and ornithine decarboxylase (2) and retained the ability to metabolize testosterone to dihydrotestosterone and other 5α-reduced metabolites (2-4).

Original languageEnglish (US)
Pages (from-to)51-54
Number of pages4
JournalJournal of Tissue Culture Methods
Volume7
Issue number2
DOIs
StatePublished - Jun 1982

Fingerprint

Canidae
Prostate
Epithelial Cells
Artificial Cells
Desmosomes
Ornithine Decarboxylase
Dihydrotestosterone
Tight Junctions
Phosphatases
Collagenases
Metabolites
Acid Phosphatase
Testosterone
Digestion
Proteins
Acids

Keywords

  • canine
  • primary culture
  • prostate
  • testosterone metabolism

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Primary culture of canine prostate epithelial cells. / Terracio, Louis; Douglas, William H J.

In: Journal of Tissue Culture Methods, Vol. 7, No. 2, 06.1982, p. 51-54.

Research output: Contribution to journalArticle

Terracio, Louis ; Douglas, William H J. / Primary culture of canine prostate epithelial cells. In: Journal of Tissue Culture Methods. 1982 ; Vol. 7, No. 2. pp. 51-54.
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