Preliminary characterization of four bacteriocins from Streptococcus mutans

M. Parrot, P. W. Caufield, M. C. Lavoie

Research output: Contribution to journalArticle

Abstract

The various properties of the inhibitory substances produced by Streptococcus mutans strains C67-1, Ny257-S, Ny266, and T8, and the fact that inhibitory zones produced could not be associated with lactic acid (or other organic acids), bacteriophages, or hydrogen peroxide indicate that these substances can be classified as mutacins. The substances produced by strains C67-1, Ny266, and T8 possessed similar properties. They were shown to be thermoresistant (100°C, 30 min), low molecular weight (<3500) substances sensitive to proteolytic enzymes (chymotrypsin, papain, pronase E, proteinase K, and trypsin) and they were active against most of the Gram-positive bacteria tested but not against most of the Gram-negative bacteria. The substance produced by strain Ny257-S differs from the other three by its narrower activity spectrum, its lower thermoresistance (80°C, 30 min), and its higher molecular weight (8000-14000). The gene or the genes coding for the mutacins are probably located on the chromosome since no plasmid DNA could be detected in these four producing strains. Restriction-fragment patterns of C67-1 and T8 suggest that these strains are closely related, as supported by the strong similarity observed between the properties of their mutacins.

Original languageEnglish (US)
Pages (from-to)123-130
Number of pages8
JournalCanadian Journal of Microbiology
Volume36
Issue number2
StatePublished - 1990

Fingerprint

Bacteriocins
Streptococcus mutans
Molecular Weight
Endopeptidase K
Pronase
Papain
Chymotrypsin
Gram-Positive Bacteria
Gram-Negative Bacteria
Bacteriophages
Trypsin
Hydrogen Peroxide
Genes
Lactic Acid
Plasmids
Peptide Hydrolases
Chromosomes
Bacteria
Acids
Molecular weight

Keywords

  • Bacteriocin
  • Inhibitory substance
  • Mutacin
  • Streptococcus mutans

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Parrot, M., Caufield, P. W., & Lavoie, M. C. (1990). Preliminary characterization of four bacteriocins from Streptococcus mutans. Canadian Journal of Microbiology, 36(2), 123-130.

Preliminary characterization of four bacteriocins from Streptococcus mutans. / Parrot, M.; Caufield, P. W.; Lavoie, M. C.

In: Canadian Journal of Microbiology, Vol. 36, No. 2, 1990, p. 123-130.

Research output: Contribution to journalArticle

Parrot, M, Caufield, PW & Lavoie, MC 1990, 'Preliminary characterization of four bacteriocins from Streptococcus mutans', Canadian Journal of Microbiology, vol. 36, no. 2, pp. 123-130.
Parrot, M. ; Caufield, P. W. ; Lavoie, M. C. / Preliminary characterization of four bacteriocins from Streptococcus mutans. In: Canadian Journal of Microbiology. 1990 ; Vol. 36, No. 2. pp. 123-130.
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