Postmeiotic unfertilized starfish eggs die by apoptosis

Research output: Contribution to journalArticle

Abstract

Fertilization of starfish eggs during meiosis results in rapid progression to embryogenesis as soon as meiosis II is completed. Unfertilized eggs complete meiosis and arrest in postmeiotic interphase for an, until now, indeterminate time. If they remain unfertilized, the mature postmeiotic eggs ultimately die. The aim of this study is to characterize the mechanism of death in postmeiotic unfertilized starfish eggs. We report that, in two species of starfish, in the absence of fertilization, postmeiotic interphase arrest persists for 16-20 h, after which time the cells synchronously and rapidly die. Dying eggs extrude membrane blebs, undergo cytoplasmic contraction and darkening, and fragment into vesicles in a manner reminiscent of apoptotic cells. The DNA of dying eggs is condensed, fragmented, and labeled by the TUNEL assay. Taken together, these data suggest that the default fate of postmeiotic starfish eggs, like their mammalian counterparts, is death by apoptosis. We further report that the onset and execution of apoptosis in this system is dependent on ongoing protein synthesis and is inhibited by a rise in intracellular Ca2+, an essential component of the fertilization signaling pathway. We propose starfish eggs as a useful model to study developmentally regulated apoptosis.

Original languageEnglish (US)
Pages (from-to)29-44
Number of pages16
JournalDevelopmental Biology
Volume237
Issue number1
DOIs
StatePublished - Sep 1 2001

Fingerprint

Starfish
Eggs
Ovum
Apoptosis
Meiosis
Fertilization
Interphase
In Situ Nick-End Labeling
Blister
Embryonic Development
Membranes
DNA

Keywords

  • Apoptosis
  • Meiotic maturation
  • Oocyte
  • Postmeiotic egg
  • Programmed cell death
  • Protein synthesis
  • Starfish.

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Postmeiotic unfertilized starfish eggs die by apoptosis. / Yuce, Ozlem; Sadler Edepli, Kirsten.

In: Developmental Biology, Vol. 237, No. 1, 01.09.2001, p. 29-44.

Research output: Contribution to journalArticle

@article{d8e68eba78ed4b27bbfe7abf0118b564,
title = "Postmeiotic unfertilized starfish eggs die by apoptosis",
abstract = "Fertilization of starfish eggs during meiosis results in rapid progression to embryogenesis as soon as meiosis II is completed. Unfertilized eggs complete meiosis and arrest in postmeiotic interphase for an, until now, indeterminate time. If they remain unfertilized, the mature postmeiotic eggs ultimately die. The aim of this study is to characterize the mechanism of death in postmeiotic unfertilized starfish eggs. We report that, in two species of starfish, in the absence of fertilization, postmeiotic interphase arrest persists for 16-20 h, after which time the cells synchronously and rapidly die. Dying eggs extrude membrane blebs, undergo cytoplasmic contraction and darkening, and fragment into vesicles in a manner reminiscent of apoptotic cells. The DNA of dying eggs is condensed, fragmented, and labeled by the TUNEL assay. Taken together, these data suggest that the default fate of postmeiotic starfish eggs, like their mammalian counterparts, is death by apoptosis. We further report that the onset and execution of apoptosis in this system is dependent on ongoing protein synthesis and is inhibited by a rise in intracellular Ca2+, an essential component of the fertilization signaling pathway. We propose starfish eggs as a useful model to study developmentally regulated apoptosis.",
keywords = "Apoptosis, Meiotic maturation, Oocyte, Postmeiotic egg, Programmed cell death, Protein synthesis, Starfish.",
author = "Ozlem Yuce and {Sadler Edepli}, Kirsten",
year = "2001",
month = "9",
day = "1",
doi = "10.1006/dbio.2001.0361",
language = "English (US)",
volume = "237",
pages = "29--44",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Postmeiotic unfertilized starfish eggs die by apoptosis

AU - Yuce, Ozlem

AU - Sadler Edepli, Kirsten

PY - 2001/9/1

Y1 - 2001/9/1

N2 - Fertilization of starfish eggs during meiosis results in rapid progression to embryogenesis as soon as meiosis II is completed. Unfertilized eggs complete meiosis and arrest in postmeiotic interphase for an, until now, indeterminate time. If they remain unfertilized, the mature postmeiotic eggs ultimately die. The aim of this study is to characterize the mechanism of death in postmeiotic unfertilized starfish eggs. We report that, in two species of starfish, in the absence of fertilization, postmeiotic interphase arrest persists for 16-20 h, after which time the cells synchronously and rapidly die. Dying eggs extrude membrane blebs, undergo cytoplasmic contraction and darkening, and fragment into vesicles in a manner reminiscent of apoptotic cells. The DNA of dying eggs is condensed, fragmented, and labeled by the TUNEL assay. Taken together, these data suggest that the default fate of postmeiotic starfish eggs, like their mammalian counterparts, is death by apoptosis. We further report that the onset and execution of apoptosis in this system is dependent on ongoing protein synthesis and is inhibited by a rise in intracellular Ca2+, an essential component of the fertilization signaling pathway. We propose starfish eggs as a useful model to study developmentally regulated apoptosis.

AB - Fertilization of starfish eggs during meiosis results in rapid progression to embryogenesis as soon as meiosis II is completed. Unfertilized eggs complete meiosis and arrest in postmeiotic interphase for an, until now, indeterminate time. If they remain unfertilized, the mature postmeiotic eggs ultimately die. The aim of this study is to characterize the mechanism of death in postmeiotic unfertilized starfish eggs. We report that, in two species of starfish, in the absence of fertilization, postmeiotic interphase arrest persists for 16-20 h, after which time the cells synchronously and rapidly die. Dying eggs extrude membrane blebs, undergo cytoplasmic contraction and darkening, and fragment into vesicles in a manner reminiscent of apoptotic cells. The DNA of dying eggs is condensed, fragmented, and labeled by the TUNEL assay. Taken together, these data suggest that the default fate of postmeiotic starfish eggs, like their mammalian counterparts, is death by apoptosis. We further report that the onset and execution of apoptosis in this system is dependent on ongoing protein synthesis and is inhibited by a rise in intracellular Ca2+, an essential component of the fertilization signaling pathway. We propose starfish eggs as a useful model to study developmentally regulated apoptosis.

KW - Apoptosis

KW - Meiotic maturation

KW - Oocyte

KW - Postmeiotic egg

KW - Programmed cell death

KW - Protein synthesis

KW - Starfish.

UR - http://www.scopus.com/inward/record.url?scp=0035448516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035448516&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0361

DO - 10.1006/dbio.2001.0361

M3 - Article

VL - 237

SP - 29

EP - 44

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -