Post-Assembly Stabilization of Rationally Designed DNA Crystals

Jiemin Zhao, Arun Richard Chandrasekaran, Qian Li, Xiang Li, Ruojie Sha, Nadrian Seeman, Chengde Mao

Research output: Contribution to journalArticle

Abstract

This manuscript reports an effort to stabilize self-assembled DNA crystals. Owing to their weak inter-unit cohesion, self-assembled DNA crystals are fragile, which limits the potential applications of such crystals. To overcome this problem, another molecule was introduced, which binds to the cohesive sites and stabilizes the inter-unit interactions. The extra interactions greatly improve the stability of the DNA crystals. The original DNA crystals are only stable in solutions of high ionic strength (e.g., ≥1.2 M (NH<inf>4</inf>)<inf>2</inf>SO<inf>4</inf>); in contrast, the stabilized crystals can be stable at ionic strengths as low as that of a 0.02M solution of (NH<inf>4</inf>)<inf>2</inf>SO<inf>4</inf>. The current strategy is expected to represent a general approach for increasing the stability of self-assembled DNA nanostructures for potential applications, for example, as structural scaffolds and molecular sieves.

Original languageEnglish (US)
Pages (from-to)9936-9939
Number of pages4
JournalAngewandte Chemie - International Edition
Volume54
Issue number34
DOIs
StatePublished - Aug 1 2015

Fingerprint

DNA
Stabilization
Crystals
Ionic strength
Molecular sieves
Scaffolds
Nanostructures
Molecules

Keywords

  • crystal engineering
  • DNA structures
  • nanostructures
  • self-assembly

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Post-Assembly Stabilization of Rationally Designed DNA Crystals. / Zhao, Jiemin; Chandrasekaran, Arun Richard; Li, Qian; Li, Xiang; Sha, Ruojie; Seeman, Nadrian; Mao, Chengde.

In: Angewandte Chemie - International Edition, Vol. 54, No. 34, 01.08.2015, p. 9936-9939.

Research output: Contribution to journalArticle

Zhao, J, Chandrasekaran, AR, Li, Q, Li, X, Sha, R, Seeman, N & Mao, C 2015, 'Post-Assembly Stabilization of Rationally Designed DNA Crystals', Angewandte Chemie - International Edition, vol. 54, no. 34, pp. 9936-9939. https://doi.org/10.1002/anie.201503610
Zhao, Jiemin ; Chandrasekaran, Arun Richard ; Li, Qian ; Li, Xiang ; Sha, Ruojie ; Seeman, Nadrian ; Mao, Chengde. / Post-Assembly Stabilization of Rationally Designed DNA Crystals. In: Angewandte Chemie - International Edition. 2015 ; Vol. 54, No. 34. pp. 9936-9939.
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