Porous bead-based diagnostic platforms

Bridging the gaps in healthcare

Jie Chou, Jorge Wong, Nicolaos Christodoulides, Pierre N. Floriano, Ximena Sanchez, John McDevitt

Research output: Contribution to journalArticle

Abstract

Advances in lab-on-a-chip systems have strong potential for multiplexed detection of a wide range of analytes with reduced sample and reagent volume; lower costs and shorter analysis times. The completion of high-fidelity multiplexed and multiclass assays remains a challenge for the medical microdevice field; as it struggles to achieve and expand upon at the point-of-care the quality of results that are achieved now routinely in remote laboratory settings. This review article serves to explore for the first time the key intersection of multiplexed bead-based detection systems with integrated microfluidic structures alongside porous capture elements together with biomarker validation studies. These strategically important elements are evaluated here in the context of platform generation as suitable for near-patient testing. Essential issues related to the scalability of these modular sensor ensembles are explored as are attempts to move such multiplexed and multiclass platforms into large-scale clinical trials. Recent efforts in these bead sensors have shown advantages over planar microarrays in terms of their capacity to generate multiplexed test results with shorter analysis times. Through high surface-to-volume ratios and encoding capabilities; porous bead-based ensembles; when combined with microfluidic elements; allow for high-throughput testing for enzymatic assays; general chemistries; protein; antibody and oligonucleotide applications.

Original languageEnglish (US)
Pages (from-to)15467-15499
Number of pages33
JournalSensors (Switzerland)
Volume12
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

Microfluidics
beads
Assays
platforms
Point-of-Care Systems
Delivery of Health Care
Lab-on-a-chip
Oligonucleotides
Validation Studies
Sensors
Enzyme Assays
Testing
Biomarkers
Microarrays
Antibodies
Scalability
systems-on-a-chip
oligonucleotides
biomarkers
sensors

Keywords

  • Beads
  • Clinical chemistry
  • Immunoassays
  • Microfluidics
  • Point-of-care
  • Porous
  • Validation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

Cite this

Chou, J., Wong, J., Christodoulides, N., Floriano, P. N., Sanchez, X., & McDevitt, J. (2012). Porous bead-based diagnostic platforms: Bridging the gaps in healthcare. Sensors (Switzerland), 12(11), 15467-15499. https://doi.org/10.3390/s121115467

Porous bead-based diagnostic platforms : Bridging the gaps in healthcare. / Chou, Jie; Wong, Jorge; Christodoulides, Nicolaos; Floriano, Pierre N.; Sanchez, Ximena; McDevitt, John.

In: Sensors (Switzerland), Vol. 12, No. 11, 11.2012, p. 15467-15499.

Research output: Contribution to journalArticle

Chou, J, Wong, J, Christodoulides, N, Floriano, PN, Sanchez, X & McDevitt, J 2012, 'Porous bead-based diagnostic platforms: Bridging the gaps in healthcare', Sensors (Switzerland), vol. 12, no. 11, pp. 15467-15499. https://doi.org/10.3390/s121115467
Chou J, Wong J, Christodoulides N, Floriano PN, Sanchez X, McDevitt J. Porous bead-based diagnostic platforms: Bridging the gaps in healthcare. Sensors (Switzerland). 2012 Nov;12(11):15467-15499. https://doi.org/10.3390/s121115467
Chou, Jie ; Wong, Jorge ; Christodoulides, Nicolaos ; Floriano, Pierre N. ; Sanchez, Ximena ; McDevitt, John. / Porous bead-based diagnostic platforms : Bridging the gaps in healthcare. In: Sensors (Switzerland). 2012 ; Vol. 12, No. 11. pp. 15467-15499.
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