Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing

Stephen Small, Susan L. Haines, Richard A. Akeson

Research output: Contribution to journalArticle

Abstract

The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%-45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.

Original languageEnglish (US)
Pages (from-to)1007-1017
Number of pages11
JournalNeuron
Volume1
Issue number10
DOIs
StatePublished - 1988

Fingerprint

Alternative Splicing
Immunoglobulins
Exons
Peptides
Brain
Amino Acids
Messenger RNA
Membrane Glycoproteins
RNA
Neurons
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing. / Small, Stephen; Haines, Susan L.; Akeson, Richard A.

In: Neuron, Vol. 1, No. 10, 1988, p. 1007-1017.

Research output: Contribution to journalArticle

@article{506eb3595d574016a5ea8ac50fccd698,
title = "Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing",
abstract = "The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3{\%}) in embryonic brain but increase postnatally to 40{\%}-45{\%} of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.",
author = "Stephen Small and Haines, {Susan L.} and Akeson, {Richard A.}",
year = "1988",
doi = "10.1016/0896-6273(88)90158-4",
language = "English (US)",
volume = "1",
pages = "1007--1017",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "10",

}

TY - JOUR

T1 - Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing

AU - Small, Stephen

AU - Haines, Susan L.

AU - Akeson, Richard A.

PY - 1988

Y1 - 1988

N2 - The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%-45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.

AB - The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%-45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.

UR - http://www.scopus.com/inward/record.url?scp=0024152699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024152699&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(88)90158-4

DO - 10.1016/0896-6273(88)90158-4

M3 - Article

VL - 1

SP - 1007

EP - 1017

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 10

ER -