Poly(N-isopropylacrylamide-co-1-vinylimidazole) hydrogel nanoparticles prepared and hydrophobically modified in liposome reactors: Atomic force microscopy and dynamic light scattering study

Sergey Kazakov, Marian Kaholek, Dina Kudasheva, Iwao Teraoka, Mary Cowman, Kalle Levon

Research output: Contribution to journalArticle

Abstract

The poly(N-isopropylacrylamide-co-1-vinylimidazole) hydrogel nanoparticles (nanogels; 30-300 nm in diameter) with hydrophobic N-octadecylacrylamide chains exposed on their surfaces have been prepared by UV-induced polymerization. The internal reservoir of liposomes was used as a container for the water-soluble components of a gel-forming medium while the interlayer space of the liposomal lipid membrane was used as a container for the water-insoluble components. The abrupt collapsing of the nanogels was accompanied with their aggregation above the volume phase transition temperature, as was observed by dynamic light scattering (DLS). The combined DLS and atomic force microscopy (AFM) study shows that the obtained hydrogel nanoparticles were highly compatible with the lipid bilayer. This compatibility led to a self-initiated coating of the hydrogel particles by a lipid layer upon mixing of liposomes and nanogels. Swollen and collapsed states of the nanogels at different pH values were visualized by AFM. The flattening of the liposomes and nanogels was compared upon their deposition onto a mica surface in air. The quantitative characteristics of the nanogels in swollen and collapsed states were revealed by cross sectioning of AFM images. The findings indicate great potential for using the spherical nanoparticles (liposomes and nanogels) as starting materials for the fabrication of planar and hemispherical biophysical nanodevices.

Original languageEnglish (US)
Pages (from-to)8086-8093
Number of pages8
JournalLangmuir
Volume19
Issue number19
DOIs
StatePublished - Sep 16 2003

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Liposomes
Hydrogel
Dynamic light scattering
Hydrogels
lipids
Atomic force microscopy
light scattering
reactors
atomic force microscopy
Nanoparticles
containers
nanoparticles
Containers
flattening
mica
compatibility
water
Lipid bilayers
interlayers
Mica

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Poly(N-isopropylacrylamide-co-1-vinylimidazole) hydrogel nanoparticles prepared and hydrophobically modified in liposome reactors : Atomic force microscopy and dynamic light scattering study. / Kazakov, Sergey; Kaholek, Marian; Kudasheva, Dina; Teraoka, Iwao; Cowman, Mary; Levon, Kalle.

In: Langmuir, Vol. 19, No. 19, 16.09.2003, p. 8086-8093.

Research output: Contribution to journalArticle

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