Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports

Patrick A. Johnson, Rastislav Levicky

Research output: Contribution to journalArticle

Abstract

A central requirement in modification of solid surfaces with biological polymers is to tether the molecule of interest permanently and in a well-defined attachment geometry. Gold is perhaps the most popular metal support for research applications, yet it suffers from a lack of methods for producing robust biomolecular films that can withstand prolonged use, especially at elevated temperatures. In this report, the stability issue is addressed by first self-assembling a nanometer thick layer of a thiol-derivatized polysiloxane, poly(mercaptopropyl)methylsiloxane (PMPMS), on the gold support. Multivalent binding of the polymer thiols to the gold, combined with the polymer's hydrophobic nature, causes it to irreversibly adhere to the metal support. Thiol-terminated, 20mer DNA oligonucleotides are subsequently covalently linked to the PMPMS film using bismaleimide cross-linkers. Immobilization coverages of up to ∼1 × 101 13 strands/cm 2 have been demonstrated. Significantly, the DNA monolayers can withstand prolonged exposure to near 100 °C conditions with minimal loss of strands from the solid support. The immobilized oligonucleotides retain ability to undergo sequence-specific hybridization, opening up applications in diagnostic and related areas.

Original languageEnglish (US)
Pages (from-to)10288-10294
Number of pages7
JournalLangmuir
Volume19
Issue number24
DOIs
StatePublished - Nov 25 2003

Fingerprint

Biomolecules
Anchors
immobilization
Sulfhydryl Compounds
Gold
Polymers
Oligonucleotides
thiols
gold
oligonucleotides
DNA
Metals
Immobilized Nucleic Acids
strands
Siloxanes
polymers
deoxyribonucleic acid
bismaleimide
Silicones
polysiloxanes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports. / Johnson, Patrick A.; Levicky, Rastislav.

In: Langmuir, Vol. 19, No. 24, 25.11.2003, p. 10288-10294.

Research output: Contribution to journalArticle

@article{104e73638f0d4778b90f2cb22bc046de,
title = "Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports",
abstract = "A central requirement in modification of solid surfaces with biological polymers is to tether the molecule of interest permanently and in a well-defined attachment geometry. Gold is perhaps the most popular metal support for research applications, yet it suffers from a lack of methods for producing robust biomolecular films that can withstand prolonged use, especially at elevated temperatures. In this report, the stability issue is addressed by first self-assembling a nanometer thick layer of a thiol-derivatized polysiloxane, poly(mercaptopropyl)methylsiloxane (PMPMS), on the gold support. Multivalent binding of the polymer thiols to the gold, combined with the polymer's hydrophobic nature, causes it to irreversibly adhere to the metal support. Thiol-terminated, 20mer DNA oligonucleotides are subsequently covalently linked to the PMPMS film using bismaleimide cross-linkers. Immobilization coverages of up to ∼1 × 101 13 strands/cm 2 have been demonstrated. Significantly, the DNA monolayers can withstand prolonged exposure to near 100 °C conditions with minimal loss of strands from the solid support. The immobilized oligonucleotides retain ability to undergo sequence-specific hybridization, opening up applications in diagnostic and related areas.",
author = "Johnson, {Patrick A.} and Rastislav Levicky",
year = "2003",
month = "11",
day = "25",
doi = "10.1021/la035102s",
language = "English (US)",
volume = "19",
pages = "10288--10294",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports

AU - Johnson, Patrick A.

AU - Levicky, Rastislav

PY - 2003/11/25

Y1 - 2003/11/25

N2 - A central requirement in modification of solid surfaces with biological polymers is to tether the molecule of interest permanently and in a well-defined attachment geometry. Gold is perhaps the most popular metal support for research applications, yet it suffers from a lack of methods for producing robust biomolecular films that can withstand prolonged use, especially at elevated temperatures. In this report, the stability issue is addressed by first self-assembling a nanometer thick layer of a thiol-derivatized polysiloxane, poly(mercaptopropyl)methylsiloxane (PMPMS), on the gold support. Multivalent binding of the polymer thiols to the gold, combined with the polymer's hydrophobic nature, causes it to irreversibly adhere to the metal support. Thiol-terminated, 20mer DNA oligonucleotides are subsequently covalently linked to the PMPMS film using bismaleimide cross-linkers. Immobilization coverages of up to ∼1 × 101 13 strands/cm 2 have been demonstrated. Significantly, the DNA monolayers can withstand prolonged exposure to near 100 °C conditions with minimal loss of strands from the solid support. The immobilized oligonucleotides retain ability to undergo sequence-specific hybridization, opening up applications in diagnostic and related areas.

AB - A central requirement in modification of solid surfaces with biological polymers is to tether the molecule of interest permanently and in a well-defined attachment geometry. Gold is perhaps the most popular metal support for research applications, yet it suffers from a lack of methods for producing robust biomolecular films that can withstand prolonged use, especially at elevated temperatures. In this report, the stability issue is addressed by first self-assembling a nanometer thick layer of a thiol-derivatized polysiloxane, poly(mercaptopropyl)methylsiloxane (PMPMS), on the gold support. Multivalent binding of the polymer thiols to the gold, combined with the polymer's hydrophobic nature, causes it to irreversibly adhere to the metal support. Thiol-terminated, 20mer DNA oligonucleotides are subsequently covalently linked to the PMPMS film using bismaleimide cross-linkers. Immobilization coverages of up to ∼1 × 101 13 strands/cm 2 have been demonstrated. Significantly, the DNA monolayers can withstand prolonged exposure to near 100 °C conditions with minimal loss of strands from the solid support. The immobilized oligonucleotides retain ability to undergo sequence-specific hybridization, opening up applications in diagnostic and related areas.

UR - http://www.scopus.com/inward/record.url?scp=0344082829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344082829&partnerID=8YFLogxK

U2 - 10.1021/la035102s

DO - 10.1021/la035102s

M3 - Article

VL - 19

SP - 10288

EP - 10294

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 24

ER -