Polymerase β simulations suggest that Arg258 rotation is a slow step rather than large subdomain motions Per Se

Linjing Yang, William A. Beard, Samuel H. Wilson, Suse Broyde, Tamar Schlick

Research output: Contribution to journalArticle

Abstract

The large-scale opening motion of mammalian DNA polymerase β is followed at atomic resolution by dynamic simulations that link crystal "closed" and "open" conformations. The closing/opening conformational change is thought to be key to the ability of polymerases to choose a correct nucleotide (through "induced fit") and hence maintain DNA repair synthesis fidelity. Corroborating available structural and kinetic measurements, our studies bridge static microscopic crystal structures with macroscopic kinetic data by delineating a specific sequence, Phe272 ring flip, large thumb movement, Arg258 rotation with release of catalytic Mg2+, together with estimated time-scales, that suggest the Arg258 rearrangement as a limiting factor of large subdomain motions. If similarly slow in the closing motion, this conformational change might be restricted further when an incorrect nucleotide binds and thus play a role in pol β's selectivity for the correct nucleotide. These results suggest new lines of experimentation in the study of polymerase mechanisms (e.g. enzyme mutants), which should provide further insights into mechanisms of error discrimination and DNA synthesis fidelity.

Original languageEnglish (US)
Pages (from-to)651-671
Number of pages21
JournalJournal of Molecular Biology
Volume317
Issue number5
DOIs
StatePublished - 2002

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Nucleotides
Thumb
DNA-Directed DNA Polymerase
DNA Repair
DNA
Enzymes

Keywords

  • Closed state
  • Dynamics simulations
  • Fidelity
  • Human DNA polymerase β
  • Open state

ASJC Scopus subject areas

  • Virology

Cite this

Polymerase β simulations suggest that Arg258 rotation is a slow step rather than large subdomain motions Per Se. / Yang, Linjing; Beard, William A.; Wilson, Samuel H.; Broyde, Suse; Schlick, Tamar.

In: Journal of Molecular Biology, Vol. 317, No. 5, 2002, p. 651-671.

Research output: Contribution to journalArticle

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