Polyhydroxybutyrate Targets Mammalian Mitochondria and Increases Permeability of Plasmalemmal and Mitochondrial Membranes

Pia A. Elustondo, Plamena R. Angelova, Michał Kawalec, Michał Michalak, Piotr Kurcok, Andrey Y. Abramov, Evgeny Pavlov

Research output: Contribution to journalArticle

Abstract

Poly(3-hydroxybutyrate) (PHB) is a polyester of 3-hydroxybutyric acid (HB) that is ubiquitously present in all organisms. In higher eukaryotes PHB is found in the length of 10 to 100 HB units and can be present in free form as well as in association with proteins and inorganic polyphosphate. It has been proposed that PHB can mediate ion transport across lipid bilayer membranes. We investigated the ability of PHB to interact with living cells and isolated mitochondria and the effects of these interactions on membrane ion transport. We performed experiments using a fluorescein derivative of PHB (fluo-PHB). We found that fluo-PHB preferentially accumulated inside the mitochondria of HeLa cells. Accumulation of fluo-PHB induced mitochondrial membrane depolarization. This membrane depolarization was significantly delayed by the inhibitor of the mitochondrial permeability transition pore - Cyclosporin A. Further experiments using intact cells as well as isolated mitochondria confirmed that the effects of PHB directly linked to its ability to facilitate ion transport, including calcium, across the membranes. We conclude that PHB demonstrates ionophoretic properties in biological membranes and this effect is most profound in mitochondria due to the selective accumulation of the polymer in this organelle.

Original languageEnglish (US)
Article numbere75812
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 23 2013

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polyhydroxybutyrate
Mitochondria
Mitochondrial Membranes
Permeability
permeability
ion transport
mitochondria
fluorescein
Fluorescein
Membranes
Ion Transport
Depolarization
Ions
Derivatives
3-hydroxybutyric acid
Hydroxybutyrates
Biological membranes
Polyphosphates
lipid bilayers
Lipid bilayers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Polyhydroxybutyrate Targets Mammalian Mitochondria and Increases Permeability of Plasmalemmal and Mitochondrial Membranes. / Elustondo, Pia A.; Angelova, Plamena R.; Kawalec, Michał; Michalak, Michał; Kurcok, Piotr; Abramov, Andrey Y.; Pavlov, Evgeny.

In: PLoS One, Vol. 8, No. 9, e75812, 23.09.2013.

Research output: Contribution to journalArticle

Elustondo, Pia A. ; Angelova, Plamena R. ; Kawalec, Michał ; Michalak, Michał ; Kurcok, Piotr ; Abramov, Andrey Y. ; Pavlov, Evgeny. / Polyhydroxybutyrate Targets Mammalian Mitochondria and Increases Permeability of Plasmalemmal and Mitochondrial Membranes. In: PLoS One. 2013 ; Vol. 8, No. 9.
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