Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions

Qi Zhang, Matti Kaisti, Alok Prabhu, Yingjie Yu, Rafael Song, Miriam H. Rafailovich, Arifur Rahman, Kalle Levon

Research output: Contribution to journalArticle

Abstract

In this study, we surface-functionalized the extended floating gate (FG) sensing surface (Al2O3) of an ion-sensitive floating-gate field-effect transistor (ISFGFET) with polyaniline/dinonylnaphthalenesulfonic acid (PANI/DNNSA) to construct an organo-surface functionalized bio-sensing device. We measured the threshold voltage (VT) of the device, which was used to determine the potential change at the interface between the PANI/DNNSA layer and aqueous solution. The device was characterized under various pHs as well as enzymatic reaction catalyzed by horseradish peroxidase (HRP) immobilized on the PANI/DNNSA functionalized floating-gate sensing surface. Interestingly, upon surface modifications, the pH and enzymatic reaction sensing behaviors of the device became dependent on the PANI/DNNSA layer, proving that open circuit potentiometric (OCP) responses can be transferred to ISFGFETs. Kinetics of the reactions was tuned through the selection of substrates, exemplified with o-Phenylenediamine (OPD) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). The potential shifts caused by those reactions were explained using an operational model that illustrates “dual sensitivity” of PANI/DNNSA toward ionic and redox species. The sensing concept for the probing of peroxidase-catalyzed charge/electron transfers can potentially be applied to hydrogen peroxide (H2O2) sensing and paves the way for multiplexed potentiometric ELISA tests on such an ion-sensitive field-effect transistor (ISFET) device.

Original languageEnglish (US)
Pages (from-to)256-264
Number of pages9
JournalElectrochimica Acta
Volume261
DOIs
StatePublished - Jan 20 2018

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Redox reactions
Polyaniline
Field effect transistors
Peroxidase
Ions
Acids
Monitoring
Ion sensitive field effect transistors
Gates (transistor)
Sulfonic Acids
Horseradish Peroxidase
Threshold voltage
Hydrogen Peroxide
Surface treatment
Hydrogen peroxide
polyaniline
Kinetics
Electrons
Networks (circuits)
Substrates

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions. / Zhang, Qi; Kaisti, Matti; Prabhu, Alok; Yu, Yingjie; Song, Rafael; Rafailovich, Miriam H.; Rahman, Arifur; Levon, Kalle.

In: Electrochimica Acta, Vol. 261, 20.01.2018, p. 256-264.

Research output: Contribution to journalArticle

Zhang, Qi ; Kaisti, Matti ; Prabhu, Alok ; Yu, Yingjie ; Song, Rafael ; Rafailovich, Miriam H. ; Rahman, Arifur ; Levon, Kalle. / Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions. In: Electrochimica Acta. 2018 ; Vol. 261. pp. 256-264.
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