Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions

Qi Zhang, Matti Kaisti, Alok Prabhu, Yingjie Yu, Rafael Song, Miriam H. Rafailovich, Arifur Rahman, Kalle Levon

    Research output: Contribution to journalArticle

    Abstract

    In this study, we surface-functionalized the extended floating gate (FG) sensing surface (Al2O3) of an ion-sensitive floating-gate field-effect transistor (ISFGFET) with polyaniline/dinonylnaphthalenesulfonic acid (PANI/DNNSA) to construct an organo-surface functionalized bio-sensing device. We measured the threshold voltage (VT) of the device, which was used to determine the potential change at the interface between the PANI/DNNSA layer and aqueous solution. The device was characterized under various pHs as well as enzymatic reaction catalyzed by horseradish peroxidase (HRP) immobilized on the PANI/DNNSA functionalized floating-gate sensing surface. Interestingly, upon surface modifications, the pH and enzymatic reaction sensing behaviors of the device became dependent on the PANI/DNNSA layer, proving that open circuit potentiometric (OCP) responses can be transferred to ISFGFETs. Kinetics of the reactions was tuned through the selection of substrates, exemplified with o-Phenylenediamine (OPD) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). The potential shifts caused by those reactions were explained using an operational model that illustrates “dual sensitivity” of PANI/DNNSA toward ionic and redox species. The sensing concept for the probing of peroxidase-catalyzed charge/electron transfers can potentially be applied to hydrogen peroxide (H2O2) sensing and paves the way for multiplexed potentiometric ELISA tests on such an ion-sensitive field-effect transistor (ISFET) device.

    Original languageEnglish (US)
    Pages (from-to)256-264
    Number of pages9
    JournalElectrochimica Acta
    Volume261
    DOIs
    StatePublished - Jan 20 2018

    Fingerprint

    Redox reactions
    Polyaniline
    Field effect transistors
    Peroxidase
    Ions
    Acids
    Monitoring
    Ion sensitive field effect transistors
    Gates (transistor)
    Sulfonic Acids
    Horseradish Peroxidase
    Threshold voltage
    Hydrogen Peroxide
    Surface treatment
    Hydrogen peroxide
    polyaniline
    Kinetics
    Electrons
    Networks (circuits)
    Substrates

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Electrochemistry

    Cite this

    Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions. / Zhang, Qi; Kaisti, Matti; Prabhu, Alok; Yu, Yingjie; Song, Rafael; Rafailovich, Miriam H.; Rahman, Arifur; Levon, Kalle.

    In: Electrochimica Acta, Vol. 261, 20.01.2018, p. 256-264.

    Research output: Contribution to journalArticle

    Zhang, Qi ; Kaisti, Matti ; Prabhu, Alok ; Yu, Yingjie ; Song, Rafael ; Rafailovich, Miriam H. ; Rahman, Arifur ; Levon, Kalle. / Polyaniline-functionalized ion-sensitive floating-gate FETs for the on-chip monitoring of peroxidase-catalyzed redox reactions. In: Electrochimica Acta. 2018 ; Vol. 261. pp. 256-264.
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    AU - Prabhu, Alok

    AU - Yu, Yingjie

    AU - Song, Rafael

    AU - Rafailovich, Miriam H.

    AU - Rahman, Arifur

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