Plasmepsin 4, the food vacuole aspartic proteinase found in all Plasmodium spp. infecting man

John B. Dame, Charles A. Yowell, Levi Omara-Opyene, Jane M. Carlton, Roland A. Cooper, Tang Li

Research output: Contribution to journalArticle

Abstract

Plasmepsins are aspartic proteinases of the malaria parasite, and seven groups of plasmepsins have been identified by comparing genomic sequence data available for the genes encoding these enzymes from Plasmodium falciparum, Plasmodium vivax, Plasmodium knowlesi, Plasmodium berghei, and Plasmodium yoelii. The food vacuole plasmepsins typified by plasmepsin 4 from P. falciparum (PfPM4) constitute one of these groups. Genes encoding the ortholog of PfPM4 have been cloned from Plasmodium ovale, Plasmodium malariae, and P. vivax. In addition, P. falciparum contains three paralagous food vacuole plasmepsins or plasmepsin-like enzymes that appear to have arisen by gene duplication, plasmepsins 1 (PfPM1), 2 (PfPM2) and HAP, and all four were localized to purified food vacuole preparations by two-dimensional gel electrophoresis and mass spectroscopic analysis. The three paralogs of PfPM4 do not have counterparts in the six other Plasmodium spp. examined by genomic DNA blot analysis and by review of available genomic sequence data. The presence of these paralogs among the food vacuole plasmepsins in P. falciparum as compared with the other three species causing malaria in man will impact efforts to rationally design antimalarials targeting the food vacuole plasmepsins.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalMolecular and Biochemical Parasitology
Volume130
Issue number1
DOIs
StatePublished - Aug 11 2003

Fingerprint

Aspartic Acid Proteases
Plasmodium
Vacuoles
Food
Plasmodium falciparum
Plasmodium vivax
Malaria
Plasmodium ovale
Plasmodium knowlesi
Plasmodium malariae
Plasmodium yoelii
plasmepsin
Plasmodium berghei
Gene Duplication
Electrophoresis, Gel, Two-Dimensional
Antimalarials
Enzymes
Genes
Parasites

Keywords

  • Aspartic proteinase
  • Food vacuole
  • Malaria
  • Multigene family
  • Ortholog
  • Paralog
  • Plasmepsin
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Plasmepsin 4, the food vacuole aspartic proteinase found in all Plasmodium spp. infecting man. / Dame, John B.; Yowell, Charles A.; Omara-Opyene, Levi; Carlton, Jane M.; Cooper, Roland A.; Li, Tang.

In: Molecular and Biochemical Parasitology, Vol. 130, No. 1, 11.08.2003, p. 1-12.

Research output: Contribution to journalArticle

Dame, John B. ; Yowell, Charles A. ; Omara-Opyene, Levi ; Carlton, Jane M. ; Cooper, Roland A. ; Li, Tang. / Plasmepsin 4, the food vacuole aspartic proteinase found in all Plasmodium spp. infecting man. In: Molecular and Biochemical Parasitology. 2003 ; Vol. 130, No. 1. pp. 1-12.
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