Plasma assisted decontamination of bacterial spores

Research output: Contribution to journalArticle

Abstract

The efficacy and mechanism of killing bacterial spores by a plasma torch is studied. Bacterial-spore (Bacillus cereus) suspension is inoculated onto glass/paper slide-coupons and desiccated into dry samples, and inoculated into well-microplate as wet sample. The exposure distance of all samples is 4 cm from the nozzle of the torch. In the experiment, paper slide-coupon is inserted inside an envelope. The kill times on spores in three types of samples are measured to be about 3, 9, and 24 seconds. The changes in the morphology and shape of still viable spores in treated wet samples are recorded by scanning electron and atomic force microscopes. The loss of appendages and exosporium in the structure and squashed/flattened cell shape are observed. The emission spectroscopy of the torch indicates that the plasma effluent carries abundant reactive atomic oxygen, which is responsible for the destruction of spores.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalThe open biomedical engineering journal
Volume2
DOIs
StatePublished - Jul 25 2008

Fingerprint

Bacterial Spores
Plasma torches
Decontamination
Spores
Bacillus cereus
Plasmas
Emission spectroscopy
Effluents
Nozzles
Suspensions
Microscopes
Cell Shape
Oxygen
Scanning
Glass
Electrons
Spectrum Analysis
Experiments

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Plasma assisted decontamination of bacterial spores. / Kuo, Spencer.

In: The open biomedical engineering journal, Vol. 2, 25.07.2008, p. 36-42.

Research output: Contribution to journalArticle

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