Pitx genes in Tunicates provide new molecular insight into the evolutionary origin of pituitary

Lionel Christiaen, Paolo Burighel, William C. Smith, Philippe Vernier, Franck Bourrat, Jean Stéphane Joly

Research output: Contribution to journalArticle

Abstract

We have initiated a project aimed at documenting molecular and cellular changes underlying the emergence of the hypothalamo-hypophyseal axis in Chordates. Considering the phylogenetic position of Tunicates and the 'pan-hypophyseal' expression pattern of Pitx genes in Vertebrate pituitary, we searched for a Pitx-related homeobox gene in the ascidian Ciona intestinalis, and identified Ci-Pitx (Ciona intestinalis Pituitary homeobox gene). We also isolated Cs-Pitx and Bs-Pitx, the Ci-Pitx respective counterparts of Ciona savignyi and Botryllus schlosseri, two other Tunicate species. Ci-Pitx mRNA encodes a putative protein exhibiting the diagnostic K50-Paired-class homeodomain and a conserved C-terminal Aristaless domain. Embryonic expression pattern of Ci-Pitx revealed a conserved expression domain in the anterior neural ridge and subsequently in the pharyngeal primordium, defined in Vertebrates as the stomodeal ectomere, which encompasses the presumptive pituitary territory. This shows that expression at early steps of pituitary development is a feature of Pitx-related genes that was already present in the last common ancestor of Chordates.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalGene
Volume287
Issue number1-2
DOIs
StatePublished - Apr 3 2002

Fingerprint

Ciona intestinalis
Urochordata
Homeobox Genes
Chordata
Genes
Vertebrates
Messenger RNA
Proteins

Keywords

  • Anterior neural ridge
  • Aristaless domain
  • Ascidian
  • Hypophysis
  • Neural complex
  • Paired-class homeodomain
  • Stomodeum

ASJC Scopus subject areas

  • Genetics

Cite this

Pitx genes in Tunicates provide new molecular insight into the evolutionary origin of pituitary. / Christiaen, Lionel; Burighel, Paolo; Smith, William C.; Vernier, Philippe; Bourrat, Franck; Joly, Jean Stéphane.

In: Gene, Vol. 287, No. 1-2, 03.04.2002, p. 107-113.

Research output: Contribution to journalArticle

Christiaen, Lionel ; Burighel, Paolo ; Smith, William C. ; Vernier, Philippe ; Bourrat, Franck ; Joly, Jean Stéphane. / Pitx genes in Tunicates provide new molecular insight into the evolutionary origin of pituitary. In: Gene. 2002 ; Vol. 287, No. 1-2. pp. 107-113.
@article{ec67b1037abe4c47991e3f568e416c65,
title = "Pitx genes in Tunicates provide new molecular insight into the evolutionary origin of pituitary",
abstract = "We have initiated a project aimed at documenting molecular and cellular changes underlying the emergence of the hypothalamo-hypophyseal axis in Chordates. Considering the phylogenetic position of Tunicates and the 'pan-hypophyseal' expression pattern of Pitx genes in Vertebrate pituitary, we searched for a Pitx-related homeobox gene in the ascidian Ciona intestinalis, and identified Ci-Pitx (Ciona intestinalis Pituitary homeobox gene). We also isolated Cs-Pitx and Bs-Pitx, the Ci-Pitx respective counterparts of Ciona savignyi and Botryllus schlosseri, two other Tunicate species. Ci-Pitx mRNA encodes a putative protein exhibiting the diagnostic K50-Paired-class homeodomain and a conserved C-terminal Aristaless domain. Embryonic expression pattern of Ci-Pitx revealed a conserved expression domain in the anterior neural ridge and subsequently in the pharyngeal primordium, defined in Vertebrates as the stomodeal ectomere, which encompasses the presumptive pituitary territory. This shows that expression at early steps of pituitary development is a feature of Pitx-related genes that was already present in the last common ancestor of Chordates.",
keywords = "Anterior neural ridge, Aristaless domain, Ascidian, Hypophysis, Neural complex, Paired-class homeodomain, Stomodeum",
author = "Lionel Christiaen and Paolo Burighel and Smith, {William C.} and Philippe Vernier and Franck Bourrat and Joly, {Jean St{\'e}phane}",
year = "2002",
month = "4",
day = "3",
doi = "10.1016/S0378-1119(01)00865-4",
language = "English (US)",
volume = "287",
pages = "107--113",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Pitx genes in Tunicates provide new molecular insight into the evolutionary origin of pituitary

AU - Christiaen, Lionel

AU - Burighel, Paolo

AU - Smith, William C.

AU - Vernier, Philippe

AU - Bourrat, Franck

AU - Joly, Jean Stéphane

PY - 2002/4/3

Y1 - 2002/4/3

N2 - We have initiated a project aimed at documenting molecular and cellular changes underlying the emergence of the hypothalamo-hypophyseal axis in Chordates. Considering the phylogenetic position of Tunicates and the 'pan-hypophyseal' expression pattern of Pitx genes in Vertebrate pituitary, we searched for a Pitx-related homeobox gene in the ascidian Ciona intestinalis, and identified Ci-Pitx (Ciona intestinalis Pituitary homeobox gene). We also isolated Cs-Pitx and Bs-Pitx, the Ci-Pitx respective counterparts of Ciona savignyi and Botryllus schlosseri, two other Tunicate species. Ci-Pitx mRNA encodes a putative protein exhibiting the diagnostic K50-Paired-class homeodomain and a conserved C-terminal Aristaless domain. Embryonic expression pattern of Ci-Pitx revealed a conserved expression domain in the anterior neural ridge and subsequently in the pharyngeal primordium, defined in Vertebrates as the stomodeal ectomere, which encompasses the presumptive pituitary territory. This shows that expression at early steps of pituitary development is a feature of Pitx-related genes that was already present in the last common ancestor of Chordates.

AB - We have initiated a project aimed at documenting molecular and cellular changes underlying the emergence of the hypothalamo-hypophyseal axis in Chordates. Considering the phylogenetic position of Tunicates and the 'pan-hypophyseal' expression pattern of Pitx genes in Vertebrate pituitary, we searched for a Pitx-related homeobox gene in the ascidian Ciona intestinalis, and identified Ci-Pitx (Ciona intestinalis Pituitary homeobox gene). We also isolated Cs-Pitx and Bs-Pitx, the Ci-Pitx respective counterparts of Ciona savignyi and Botryllus schlosseri, two other Tunicate species. Ci-Pitx mRNA encodes a putative protein exhibiting the diagnostic K50-Paired-class homeodomain and a conserved C-terminal Aristaless domain. Embryonic expression pattern of Ci-Pitx revealed a conserved expression domain in the anterior neural ridge and subsequently in the pharyngeal primordium, defined in Vertebrates as the stomodeal ectomere, which encompasses the presumptive pituitary territory. This shows that expression at early steps of pituitary development is a feature of Pitx-related genes that was already present in the last common ancestor of Chordates.

KW - Anterior neural ridge

KW - Aristaless domain

KW - Ascidian

KW - Hypophysis

KW - Neural complex

KW - Paired-class homeodomain

KW - Stomodeum

UR - http://www.scopus.com/inward/record.url?scp=0037012540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037012540&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(01)00865-4

DO - 10.1016/S0378-1119(01)00865-4

M3 - Article

C2 - 11992728

AN - SCOPUS:0037012540

VL - 287

SP - 107

EP - 113

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -