Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation

Paulo Coelho, Maria E. Coimbra, Cristiane Ribeiro, Elizabeth Fancio, Olga Higa, Marcelo Suzuki, Mauro Marincola

Research output: Contribution to journalArticle

Abstract

This study aimed to physico/chemically characterize and evaluate the in vivo performance of a β-TCP particulate grafting material. SEM/TEM, and EDS and XPS were used for morphology and chemistry assessment, respectively. FTIR was used to determine Ca-P phases characteristic bands. Rietveld refinement/XRD spectra was performed for secondary phase detection. Particle size distribution and specific surface were assessed by a scattering-laser based technique and BET, respectively. Mercury porosimetry was employed to determine pore-size distribution. For in vivo evaluation, the grafting material was used in 12 patients' sinus lifts, and biopsies were obtained at post-operative times of 3, 6, and 9 months. SEM/TEM revealed multigrained particles with interconnected pores. EDS showed Ca, P, and O, with stoichiometry close to theoretical values. XRD/Rietveld showed that the material presented crystalline β-TCP with ~ 9% β-Ca2P2O7 secondary phase. FTIR did not detect the presence of bands related to α-TCP. Human histologic assessment showed that newly formed bone was present at 3 months, and degrees of bone organization increased as time elapsed in vivo. Human histology showed that the material is suitable for bone regeneration in a maxillofacial complex region.

Original languageEnglish (US)
Pages (from-to)2085-2091
Number of pages7
JournalMaterials Science and Engineering C
Volume29
Issue number7
DOIs
StatePublished - Aug 31 2009

Fingerprint

Histology
histology
particulates
bones
Bone
augmentation
Energy dispersive spectroscopy
Transmission electron microscopy
porosity
sinuses
transmission electron microscopy
Scanning electron microscopy
scanning electron microscopy
Rietveld refinement
Biopsy
particle size distribution
Mercury
regeneration
Stoichiometry
Particle size analysis

Keywords

  • β-TCP
  • Biocompatibility
  • Human histology
  • In vitro test
  • Physicho/chemical characterization

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation. / Coelho, Paulo; Coimbra, Maria E.; Ribeiro, Cristiane; Fancio, Elizabeth; Higa, Olga; Suzuki, Marcelo; Marincola, Mauro.

In: Materials Science and Engineering C, Vol. 29, No. 7, 31.08.2009, p. 2085-2091.

Research output: Contribution to journalArticle

Coelho, Paulo ; Coimbra, Maria E. ; Ribeiro, Cristiane ; Fancio, Elizabeth ; Higa, Olga ; Suzuki, Marcelo ; Marincola, Mauro. / Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation. In: Materials Science and Engineering C. 2009 ; Vol. 29, No. 7. pp. 2085-2091.
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