Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U

Maya Ameyar-Zazoua, Mouloud Souidi, Lauriane Fritsch, Philippe Robin, Audrey Thomas, Ali Hamiche, Piergiorgio Percipalle, Slimane Ait-Si-Ali, Annick Harel-Bellan

    Research output: Contribution to journalArticle

    Abstract

    By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

    Original languageEnglish (US)
    Pages (from-to)27974-27979
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume284
    Issue number41
    DOIs
    StatePublished - Oct 9 2009

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    Heterogeneous-Nuclear Ribonucleoprotein U
    RNA-Binding Proteins
    Chromatin
    Gene Silencing
    Purification
    Mass spectrometry
    heterochromatin-specific nonhistone chromosomal protein HP-1
    Mass Spectrometry
    Proteins
    Genes
    Molecules

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U. / Ameyar-Zazoua, Maya; Souidi, Mouloud; Fritsch, Lauriane; Robin, Philippe; Thomas, Audrey; Hamiche, Ali; Percipalle, Piergiorgio; Ait-Si-Ali, Slimane; Harel-Bellan, Annick.

    In: Journal of Biological Chemistry, Vol. 284, No. 41, 09.10.2009, p. 27974-27979.

    Research output: Contribution to journalArticle

    Ameyar-Zazoua, M, Souidi, M, Fritsch, L, Robin, P, Thomas, A, Hamiche, A, Percipalle, P, Ait-Si-Ali, S & Harel-Bellan, A 2009, 'Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U', Journal of Biological Chemistry, vol. 284, no. 41, pp. 27974-27979. https://doi.org/10.1074/jbc.M109.037929
    Ameyar-Zazoua, Maya ; Souidi, Mouloud ; Fritsch, Lauriane ; Robin, Philippe ; Thomas, Audrey ; Hamiche, Ali ; Percipalle, Piergiorgio ; Ait-Si-Ali, Slimane ; Harel-Bellan, Annick. / Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 41. pp. 27974-27979.
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    AU - Souidi, Mouloud

    AU - Fritsch, Lauriane

    AU - Robin, Philippe

    AU - Thomas, Audrey

    AU - Hamiche, Ali

    AU - Percipalle, Piergiorgio

    AU - Ait-Si-Ali, Slimane

    AU - Harel-Bellan, Annick

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