Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria

Pia A. Elustondo, Adrienne E. White, Meghan E. Hughes, Karen Brebner, Evgeny Pavlov, Daniel A. Kane

Research output: Contribution to journalArticle

Abstract

Background: The notion of mitochondrial lactate oxidation in skeletal muscle is controversial. Results: Mitochondrial respiration increased in the presence of substrates and cofactors for the lactate dehydrogenase (LDH) reaction. Respiration was inhibited when mitochondrial pyruvate transport was blocked. Conclusion: Extra-matrix LDH is associated with muscle mitochondria. Significance: LDH is strategically positioned within skeletal muscle fibers to functionally interact with mitochondria.

Original languageEnglish (US)
Pages (from-to)25309-25317
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number35
DOIs
StatePublished - Aug 30 2013

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Muscle Mitochondrion
Mitochondria
L-Lactate Dehydrogenase
Muscle
Skeletal Muscle
Association reactions
Respiration
Skeletal Muscle Fibers
Pyruvic Acid
Lactic Acid
Oxidation
Fibers
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria. / Elustondo, Pia A.; White, Adrienne E.; Hughes, Meghan E.; Brebner, Karen; Pavlov, Evgeny; Kane, Daniel A.

In: Journal of Biological Chemistry, Vol. 288, No. 35, 30.08.2013, p. 25309-25317.

Research output: Contribution to journalArticle

Elustondo, Pia A. ; White, Adrienne E. ; Hughes, Meghan E. ; Brebner, Karen ; Pavlov, Evgeny ; Kane, Daniel A. / Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 35. pp. 25309-25317.
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