Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling

Rajan Thakur, Aniruddha Panda, Elise Coessens, Nikita Raj, Shweta Yadav, Sruthi Balakrishnan, Qifeng Zhang, Plamen Georgiev, Bishal Basak, Renu Pasricha, Michael J.O. Wakelam, Nicholas T. Ktistakis, Padinjat Raghu

Research output: Contribution to journalArticle

Abstract

During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an enhanced reduction in rhabdomere size, accumulation of Rab7 positive, rhodopsin1-containing vesicles (RLVs) in the cell body and reduced rhodopsin protein. These phenotypes were associated with reduced levels of phosphatidic acid, the product of PLD activity and were rescued by reconstitution with catalytically active PLD. In wild-type photoreceptors, during illumination, enhanced PLD activity was sufficient to clear RLVs from the cell body by a process dependent on Arf1-GTP levels and retromer complex function. Thus, during illumination, PLD activity couples endocytosis of RLVs with their recycling to the plasma membrane thus maintaining plasma membrane size and composition.

Original languageEnglish (US)
Article numbere18515
JournaleLife
Volume5
Issue numberNOVEMBER2016
DOIs
StatePublished - Nov 16 2016

Fingerprint

Phospholipase D
Recycling
Cell membranes
Endocytosis
Lighting
Cell Membrane
Cells
Light
Phosphatidic Acids
Rhodopsin
Guanosine Triphosphate
Chemical analysis
Drosophila
Phenotype
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Thakur, R., Panda, A., Coessens, E., Raj, N., Yadav, S., Balakrishnan, S., ... Raghu, P. (2016). Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. eLife, 5(NOVEMBER2016), [e18515]. https://doi.org/10.7554/eLife.18515

Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. / Thakur, Rajan; Panda, Aniruddha; Coessens, Elise; Raj, Nikita; Yadav, Shweta; Balakrishnan, Sruthi; Zhang, Qifeng; Georgiev, Plamen; Basak, Bishal; Pasricha, Renu; Wakelam, Michael J.O.; Ktistakis, Nicholas T.; Raghu, Padinjat.

In: eLife, Vol. 5, No. NOVEMBER2016, e18515, 16.11.2016.

Research output: Contribution to journalArticle

Thakur, R, Panda, A, Coessens, E, Raj, N, Yadav, S, Balakrishnan, S, Zhang, Q, Georgiev, P, Basak, B, Pasricha, R, Wakelam, MJO, Ktistakis, NT & Raghu, P 2016, 'Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling', eLife, vol. 5, no. NOVEMBER2016, e18515. https://doi.org/10.7554/eLife.18515
Thakur R, Panda A, Coessens E, Raj N, Yadav S, Balakrishnan S et al. Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. eLife. 2016 Nov 16;5(NOVEMBER2016). e18515. https://doi.org/10.7554/eLife.18515
Thakur, Rajan ; Panda, Aniruddha ; Coessens, Elise ; Raj, Nikita ; Yadav, Shweta ; Balakrishnan, Sruthi ; Zhang, Qifeng ; Georgiev, Plamen ; Basak, Bishal ; Pasricha, Renu ; Wakelam, Michael J.O. ; Ktistakis, Nicholas T. ; Raghu, Padinjat. / Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. In: eLife. 2016 ; Vol. 5, No. NOVEMBER2016.
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abstract = "During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an enhanced reduction in rhabdomere size, accumulation of Rab7 positive, rhodopsin1-containing vesicles (RLVs) in the cell body and reduced rhodopsin protein. These phenotypes were associated with reduced levels of phosphatidic acid, the product of PLD activity and were rescued by reconstitution with catalytically active PLD. In wild-type photoreceptors, during illumination, enhanced PLD activity was sufficient to clear RLVs from the cell body by a process dependent on Arf1-GTP levels and retromer complex function. Thus, during illumination, PLD activity couples endocytosis of RLVs with their recycling to the plasma membrane thus maintaining plasma membrane size and composition.",
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