Phase sensitive diffraction sensor for high sensitivity refractive index measurement

Nityanand Kumawat, Manoj Varma, Sunil Kumar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study a diffraction based sensor has been developed for bio molecular sensing applications and performing assays in real time. A diffraction grating fabricated on a glass substrate produced diffraction patterns both in transmission and reflection when illuminated by a laser diode. We used zeroth order I(0,0) as reference and first order I(0,1) as signal channel and conducted ratiometric measurements that reduced noise by more than 50 times. The ratiometric approach resulted in a very simple instrumentation with very high sensitivity. In the past, we have shown refractive index measurements both for bulk and surface adsorption using the diffractive self-referencing approach. In the current work we extend the same concept to higher diffraction orders. We have considered order I(0,1) and I(1,1) and performed ratiometric measurements I(0,1)/I(1,1) to eliminate the common mode fluctuations. Since orders I(0,1) and I(1,1) behaved opposite to each other, the resulting ratio signal amplitude increased more than twice compared to our previous results. As a proof of concept we used different salt concentrations in DI water. Increased signal amplitude and improved fluid injection system resulted in more than 4 times improvement in detection limit, giving limit of detection 1.3×10-7 refractive index unit (RIU) compared to our previous results. The improved refractive index sensitivity will help significantly for high sensitivity label free bio sensing application in a very cost-effective and simple experimental set-up.

Original languageEnglish (US)
Title of host publicationOptical Diagnostics and Sensing XVIII
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
PublisherSPIE
Volume10501
ISBN (Electronic)9781510614871
DOIs
StatePublished - Jan 1 2018
EventOptical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics 2018 - San Francisco, United States
Duration: Jan 29 2018Jan 30 2018

Other

OtherOptical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics 2018
CountryUnited States
CitySan Francisco
Period1/29/181/30/18

Fingerprint

Refractometry
Refractive index
Diffraction
refractivity
Limit of Detection
sensitivity
sensors
Sensors
diffraction
Semiconductor Lasers
Diffraction gratings
fluid injection
Diffraction patterns
Adsorption
Glass
Semiconductor lasers
Noise
Labels
Assays
gratings (spectra)

Keywords

  • Diffraction grating
  • Label-Free
  • Optical Biosensor
  • Ratiometric
  • Refractive Index
  • Self-Referencing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kumawat, N., Varma, M., & Kumar, S. (2018). Phase sensitive diffraction sensor for high sensitivity refractive index measurement. In G. L. Cote (Ed.), Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics (Vol. 10501). [105010C] SPIE. https://doi.org/10.1117/12.2289740

Phase sensitive diffraction sensor for high sensitivity refractive index measurement. / Kumawat, Nityanand; Varma, Manoj; Kumar, Sunil.

Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics. ed. / Gerard L. Cote. Vol. 10501 SPIE, 2018. 105010C.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kumawat, N, Varma, M & Kumar, S 2018, Phase sensitive diffraction sensor for high sensitivity refractive index measurement. in GL Cote (ed.), Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics. vol. 10501, 105010C, SPIE, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics 2018, San Francisco, United States, 1/29/18. https://doi.org/10.1117/12.2289740
Kumawat N, Varma M, Kumar S. Phase sensitive diffraction sensor for high sensitivity refractive index measurement. In Cote GL, editor, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics. Vol. 10501. SPIE. 2018. 105010C https://doi.org/10.1117/12.2289740
Kumawat, Nityanand ; Varma, Manoj ; Kumar, Sunil. / Phase sensitive diffraction sensor for high sensitivity refractive index measurement. Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics. editor / Gerard L. Cote. Vol. 10501 SPIE, 2018.
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