PH-dependent conformational ensemble and polymorphism of amyloid-β core fragment

Weixin Xu, Ce Zhang, Ludmilla Morozova-Roche, John Zhang, Yuguang Mu

Research output: Contribution to journalArticle

Abstract

Characterization of amyloid oligomeric species is important due to its possible responsibility for the toxicity of amyloid proteins, whereas it is difficult to detect by current spectroscopic techniques. The pH-dependent tetramerization and fibrillation of the central hydrophobic segment of Alzheimer amyloid β-peptide (Aβ12-24) were respectively explored by all-atom replica exchange molecular dynamics simulations and by fluorescence and atomic force microscopy measurements. Our combined study shows that more β-sheet structures in the early event of tetramerization is linked directly to the high propensity to form amyloid fibrils in the consequent fibrillation. Both tetramerization and fibrillation are strongly regulated by pH. At pH 5.0, Aβ12-24 has two opposite terminal charges. The electrostatic attraction between the side-chains of His13/His14 and Glu22/Asp23 thus acts as a "pattern keeper", resulting in high propensity of amyloid formation. These results suggest that pH effects most likely by affecting the ionization properties of the Aβ12-24 peptide. Specifically, the pH-dependent equilibrium conformational distribution of different aggregate species are well-investigated in detail. Our findings also give hints to other experimental findings that the kinetics and morphologies of Aβ fibril formation are strongly pH-dependent.

Original languageEnglish (US)
Pages (from-to)8392-8399
Number of pages8
JournalJournal of Physical Chemistry B
Volume117
Issue number28
DOIs
StatePublished - Jul 18 2013

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polymorphism
Polymorphism
Amyloid
fragments
fibrillation
Peptides
pH effects
Amyloidogenic Proteins
peptides
Ionization
Toxicity
Molecular dynamics
Electrostatics
Atomic force microscopy
Ion exchange
Fluorescence
replicas
toxicity
Atoms
Kinetics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

PH-dependent conformational ensemble and polymorphism of amyloid-β core fragment. / Xu, Weixin; Zhang, Ce; Morozova-Roche, Ludmilla; Zhang, John; Mu, Yuguang.

In: Journal of Physical Chemistry B, Vol. 117, No. 28, 18.07.2013, p. 8392-8399.

Research output: Contribution to journalArticle

Xu, Weixin ; Zhang, Ce ; Morozova-Roche, Ludmilla ; Zhang, John ; Mu, Yuguang. / PH-dependent conformational ensemble and polymorphism of amyloid-β core fragment. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 28. pp. 8392-8399.
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