PFG-ω1-Filtered TOCSY Experiments for the Determination of Long-Range Heteronuclear and Homonuclear Coupling Constants and Estimation of J-Coupling "Crosstalk" Artifacts in 2-D ω1-Filtered "E. COSY-Style" Spectra

Guangzhao Xu, Bo Zhang, John Evans

Research output: Contribution to journalArticle

Abstract

We present novel one- and two-dimensional versions of the ω1-filtered TOCSY experiment. These experiments utilize pulsed-field gradient techniques and INEPT-reverse INEPT magnetization transfer to generate heteronuclear filtering by means of coherence pathway selection. The major advantages of this approach are twofold: first, each experiment requires a reasonable number of transmitter pulses, gradient pulses, and delays to implement. Second, the use of z-axis gradients at the beginning and termination of the pulse sequences prevents the recovery of dephased magnetization prior to FID detection. This technique was incorporated into 1-D and 2-D ω1-filtered JXH- and JHH-TOCSY-style experiments. As demonstrated on 15N-enriched peptide samples, the use of the pulsed-field-gradient coherence selection scheme effectively filters out unwanted magnetization components, thereby improving the overall sensitivity of the experiments. In addition to this suite of pulse sequences, we also present a method for correcting the reduction in J-coupling that results from crosspeak shifting in 2-D ω1-filtered E. COSY-style spectra. This correction is applicable to both Lorentzian and Gaussian 2-D crosspeak lineshapes.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalJournal of Magnetic Resonance
Volume138
Issue number1
StatePublished - May 1999

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Crosstalk
crosstalk
Artifacts
artifacts
gradients
Peptides
pulses
Magnetization
magnetization
Experiments
transmitters
peptides
recovery
Transmitters
filters
sensitivity
Recovery

Keywords

  • Crosstalk J-coupling correction
  • Crosstalk relaxation
  • Heteronuclear
  • Homonuclear
  • Isotropic mixing
  • J-coupling
  • Peptides
  • Pulsed-field gradient

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

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title = "PFG-ω1-Filtered TOCSY Experiments for the Determination of Long-Range Heteronuclear and Homonuclear Coupling Constants and Estimation of J-Coupling {"}Crosstalk{"} Artifacts in 2-D ω1-Filtered {"}E. COSY-Style{"} Spectra",
abstract = "We present novel one- and two-dimensional versions of the ω1-filtered TOCSY experiment. These experiments utilize pulsed-field gradient techniques and INEPT-reverse INEPT magnetization transfer to generate heteronuclear filtering by means of coherence pathway selection. The major advantages of this approach are twofold: first, each experiment requires a reasonable number of transmitter pulses, gradient pulses, and delays to implement. Second, the use of z-axis gradients at the beginning and termination of the pulse sequences prevents the recovery of dephased magnetization prior to FID detection. This technique was incorporated into 1-D and 2-D ω1-filtered JXH- and JHH-TOCSY-style experiments. As demonstrated on 15N-enriched peptide samples, the use of the pulsed-field-gradient coherence selection scheme effectively filters out unwanted magnetization components, thereby improving the overall sensitivity of the experiments. In addition to this suite of pulse sequences, we also present a method for correcting the reduction in J-coupling that results from crosspeak shifting in 2-D ω1-filtered E. COSY-style spectra. This correction is applicable to both Lorentzian and Gaussian 2-D crosspeak lineshapes.",
keywords = "Crosstalk J-coupling correction, Crosstalk relaxation, Heteronuclear, Homonuclear, Isotropic mixing, J-coupling, Peptides, Pulsed-field gradient",
author = "Guangzhao Xu and Bo Zhang and John Evans",
year = "1999",
month = "5",
language = "English (US)",
volume = "138",
pages = "127--134",
journal = "Journal of Magnetic Resonance",
issn = "1090-7807",
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TY - JOUR

T1 - PFG-ω1-Filtered TOCSY Experiments for the Determination of Long-Range Heteronuclear and Homonuclear Coupling Constants and Estimation of J-Coupling "Crosstalk" Artifacts in 2-D ω1-Filtered "E. COSY-Style" Spectra

AU - Xu, Guangzhao

AU - Zhang, Bo

AU - Evans, John

PY - 1999/5

Y1 - 1999/5

N2 - We present novel one- and two-dimensional versions of the ω1-filtered TOCSY experiment. These experiments utilize pulsed-field gradient techniques and INEPT-reverse INEPT magnetization transfer to generate heteronuclear filtering by means of coherence pathway selection. The major advantages of this approach are twofold: first, each experiment requires a reasonable number of transmitter pulses, gradient pulses, and delays to implement. Second, the use of z-axis gradients at the beginning and termination of the pulse sequences prevents the recovery of dephased magnetization prior to FID detection. This technique was incorporated into 1-D and 2-D ω1-filtered JXH- and JHH-TOCSY-style experiments. As demonstrated on 15N-enriched peptide samples, the use of the pulsed-field-gradient coherence selection scheme effectively filters out unwanted magnetization components, thereby improving the overall sensitivity of the experiments. In addition to this suite of pulse sequences, we also present a method for correcting the reduction in J-coupling that results from crosspeak shifting in 2-D ω1-filtered E. COSY-style spectra. This correction is applicable to both Lorentzian and Gaussian 2-D crosspeak lineshapes.

AB - We present novel one- and two-dimensional versions of the ω1-filtered TOCSY experiment. These experiments utilize pulsed-field gradient techniques and INEPT-reverse INEPT magnetization transfer to generate heteronuclear filtering by means of coherence pathway selection. The major advantages of this approach are twofold: first, each experiment requires a reasonable number of transmitter pulses, gradient pulses, and delays to implement. Second, the use of z-axis gradients at the beginning and termination of the pulse sequences prevents the recovery of dephased magnetization prior to FID detection. This technique was incorporated into 1-D and 2-D ω1-filtered JXH- and JHH-TOCSY-style experiments. As demonstrated on 15N-enriched peptide samples, the use of the pulsed-field-gradient coherence selection scheme effectively filters out unwanted magnetization components, thereby improving the overall sensitivity of the experiments. In addition to this suite of pulse sequences, we also present a method for correcting the reduction in J-coupling that results from crosspeak shifting in 2-D ω1-filtered E. COSY-style spectra. This correction is applicable to both Lorentzian and Gaussian 2-D crosspeak lineshapes.

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KW - Homonuclear

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KW - Pulsed-field gradient

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