Persistent protein kinase activation in the maintenance phase of long-term potentiation

Eric Klann, Shu Jen Chen, J. David Sweatt

Research output: Contribution to journalArticle

Abstract

Long-term potentiation (LTP) of synaptic transmission in the hippocampus is a robust form of synaptic plasticity that may contribute to mammalian memory formation. A variety of pharmacological evidence suggests that persistent kinase activation contributes to the maintenance of LTP. To determine whether persistent activation of protein kinases was associated with the maintenance phase of LTP, protein kinase activity was measured in control and LTP samples using exogenous protein kinase substrates in an in vitro assay of homogenates of the CAl region of rat hippocampal slices. After LTP, protein kinase activity was persistently increased, and the induction of this effect was blocked by the N-methyl-D-aspartate receptor antagonist DL-2-amino-5-phosphonovaleric acid. The increased protein kinase activity was found to be significantly attenuated by PKC(19-36), a selective peptide inhibitor of protein kinase C. Thus, LTP is associated with an N-methyl-D-aspartate receptor-mediated generation of a persistently activated form of protein kinase C. These data lend strong support to the model that persistent protein kinase activation contributes to the maintenance of LTP.

Original languageEnglish (US)
Pages (from-to)24253-24256
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number36
StatePublished - Dec 25 1991

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Long-Term Potentiation
Protein Kinases
Chemical activation
Maintenance
N-Methyl-D-Aspartate Receptors
Protein Kinase C
2-Amino-5-phosphonovalerate
Neuronal Plasticity
Peptides
Plasticity
Rats
Assays
Synaptic Transmission
Phosphotransferases
Hippocampus
Data storage equipment
Pharmacology
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Persistent protein kinase activation in the maintenance phase of long-term potentiation. / Klann, Eric; Chen, Shu Jen; Sweatt, J. David.

In: Journal of Biological Chemistry, Vol. 266, No. 36, 25.12.1991, p. 24253-24256.

Research output: Contribution to journalArticle

Klann, Eric ; Chen, Shu Jen ; Sweatt, J. David. / Persistent protein kinase activation in the maintenance phase of long-term potentiation. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 36. pp. 24253-24256.
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