Peripheral endothelin A receptor antagonism attenuates carcinoma-induced pain

Brian Schmidt, Victoria Pickering, Stanley Liu, Phuong Quang, John Dolan, S. Thaddeus Connelly, Richard C K Jordan

Research output: Contribution to journalArticle

Abstract

In this study we investigated the role of endothelin-1 (ET-1) and its peripheral receptor (ET-A) in carcinoma-induced pain in a mouse cancer pain model. Tumors were induced in the hind paw of female mice by local injection of cells derived from a human oral squamous cell carcinoma (SCC). Significant pain, as indicated by reduction in withdrawal thresholds in response to mechanical stimulation, began at four days after SCC inoculation and lasted to 28 days, the last day of measurement. Intra-tumor expression of both ET-1 mRNA and ET-1 protein were significantly upregulated compared to normal tissue, and local administration of the ET-A receptor selective antagonist, BQ-123 (100 μM) significantly elevated withdrawal thresholds, indicating the induction of an antinociceptive effect. These findings support the suggestion that ET-1 and ET-A receptors contribute to the severity of carcinoma-induced soft tissue cancer pain.

Original languageEnglish (US)
Pages (from-to)406-414
Number of pages9
JournalEuropean Journal of Pain
Volume11
Issue number4
DOIs
StatePublished - May 2007

Fingerprint

Endothelin A Receptors
Endothelin-1
Carcinoma
Pain
Squamous Cell Carcinoma
Nociceptive Pain
Neoplasms
Messenger RNA
Injections
Proteins
Cancer Pain

Keywords

  • Cancer pain
  • Endothelin
  • Endothelin A receptor
  • Mouse model
  • Oral cancer

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Neurology
  • Neuropsychology and Physiological Psychology

Cite this

Schmidt, B., Pickering, V., Liu, S., Quang, P., Dolan, J., Connelly, S. T., & Jordan, R. C. K. (2007). Peripheral endothelin A receptor antagonism attenuates carcinoma-induced pain. European Journal of Pain, 11(4), 406-414. https://doi.org/10.1016/j.ejpain.2006.05.007

Peripheral endothelin A receptor antagonism attenuates carcinoma-induced pain. / Schmidt, Brian; Pickering, Victoria; Liu, Stanley; Quang, Phuong; Dolan, John; Connelly, S. Thaddeus; Jordan, Richard C K.

In: European Journal of Pain, Vol. 11, No. 4, 05.2007, p. 406-414.

Research output: Contribution to journalArticle

Schmidt, B, Pickering, V, Liu, S, Quang, P, Dolan, J, Connelly, ST & Jordan, RCK 2007, 'Peripheral endothelin A receptor antagonism attenuates carcinoma-induced pain', European Journal of Pain, vol. 11, no. 4, pp. 406-414. https://doi.org/10.1016/j.ejpain.2006.05.007
Schmidt, Brian ; Pickering, Victoria ; Liu, Stanley ; Quang, Phuong ; Dolan, John ; Connelly, S. Thaddeus ; Jordan, Richard C K. / Peripheral endothelin A receptor antagonism attenuates carcinoma-induced pain. In: European Journal of Pain. 2007 ; Vol. 11, No. 4. pp. 406-414.
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