Periluminal distribution of HIV-binding target cells and Gp340 in the oral, cervical and sigmoid/rectal mucosae: A mapping study

Mariia Patyka, Daniel Malamud, Drew Weissman, William R. Abrams, Zoya Kurago

Research output: Contribution to journalArticle

Abstract

Studies have shown that the transmission of HIV is most likely to occur via rectal or vaginal routes, and rarely through oral exposure. However, the mechanisms of virus entry at mucosal surfaces remain incompletely understood. Prophylactic strategies against HIV infection may be attainable once gaps in current knowledge are filled. To address these gaps, we evaluated essentially normal epithelial surfaces and mapped the periluminal distribution of CD4<sup>+</sup> HIV target cells, including T cells and antigen-presenting cells, and an HIV-binding molecule gp340 that can be expressed by epithelial cells in secreted and cell-associated forms. Immunohistochemistry for CD4, CD16, CD3, CD1a and gp340 in human oral, rectal/ sigmoid and cervical mucosal samples from HIV-negative subjects demonstrated that periluminal HIV target cells were more prevalent at rectal/sigmoid and endocervical surfaces lined by simple columnar epithelium, than at oral and ectocervical surfaces covered by multilayered stratified squamous epithelium (p<0.001). gp340 expression patterns at these sites were also distinct and strong in oral minor salivary gland acini and ducts, including ductal saliva, in individual rectum/sigmoid and endocervix periluminar columnar cells, and in ectocervix squamous cells. Only weak expression was noted in the oral non-ductal squamous epithelium. We conclude that periluminal HIV target cells, together with periluminal epithelial cell-associated gp340 appear to be most accessible for HIV transmission at rectal/ sigmoid and endocervical surfaces. Our data help define vulnerable structural features of mucosal sites exposed to HIV.

Original languageEnglish (US)
Article numbere0132942
JournalPLoS One
Volume10
Issue number7
DOIs
StatePublished - Jul 14 2015

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Sigmoid Colon
mucosa
mouth
Mucous Membrane
HIV
epithelium
cells
Epithelium
Epithelial Cells
epithelial cells
T-cells
Viruses
antigen-presenting cells
Ducts
HIV infections
rectum
Salivary Ducts
salivary glands
saliva
Minor Salivary Glands

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Periluminal distribution of HIV-binding target cells and Gp340 in the oral, cervical and sigmoid/rectal mucosae : A mapping study. / Patyka, Mariia; Malamud, Daniel; Weissman, Drew; Abrams, William R.; Kurago, Zoya.

In: PLoS One, Vol. 10, No. 7, e0132942, 14.07.2015.

Research output: Contribution to journalArticle

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