Peak levels of BMP in the Drosophila embryo control target genes by a feed-forward mechanism

Mu Xu, Nikolai Kirov, Christine Rushlow

Research output: Contribution to journalArticle

Abstract

Gradients of morphogens determine cell fates by specifying discrete thresholds of gene activities. In the Drosophila embryo, a BMP gradient subdivides the dorsal ectoderm into amnioserosa and dorsal epidermis, and also inhibits neuroectoderm formation. A number of genes are differentially expressed in response to the gradient, but how their borders of expression are established is not well understood. We present evidence that the BMP gradient, via the Smads, provides a two-fold input in regulating the amnioserosa-specific target genes such as Race. Peak levels of Smads in the presumptive amnioserosa set the expression domain of zen, and then Smads act in combination with Zen to directly activate Race. This situation resembles a feed-forward mechanism of transcriptional regulation. In addition, we demonstrate that ectopically expressed Zen can activate targets like Race in the presence of low level Smads, indicating that the role of the highest activity of the BMP gradient is to activate zen.

Original languageEnglish (US)
Pages (from-to)1637-1647
Number of pages11
JournalDevelopment
Volume132
Issue number7
DOIs
StatePublished - Apr 2005

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Drosophila
Embryonic Structures
Genes
Neural Plate
Ectoderm
Epidermis

Keywords

  • BMP gradient
  • Feed-forward
  • Race
  • Zen

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Peak levels of BMP in the Drosophila embryo control target genes by a feed-forward mechanism. / Xu, Mu; Kirov, Nikolai; Rushlow, Christine.

In: Development, Vol. 132, No. 7, 04.2005, p. 1637-1647.

Research output: Contribution to journalArticle

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