Patterns of nucleotide variation in homoeologous regulatory genes in the allotetraploid Hawaiian silversword alliance (Asteraceae)

Amy Lawton-Rauh, Robert H. Robichaux, Michael D. Purugganan

Research output: Contribution to journalArticle

Abstract

Genome-wide duplication (polyploidization) is prevalent in a large number of eukaryotic organisms and is particularly widespread in flowering plants. Polyploid species appear to vary from their diploid progenitors in a variety of ecologically important traits, suggesting that genome duplications provide a mechanism for ecological diversification. Studies of nucleotide variation at duplicate genes that arise via polyploidization allow us to infer the evolutionary forces that act on these polyploid loci. In an effort to examine the evolutionary dynamics of homoeologous loci, molecular population genetic analyses were undertaken for duplicate regulatory genes in the allopolyploid Hawaiian silversword alliance, a premier example of adaptive radiation. The levels and patterns of nucleotide variation for the floral homeotic genes ASAPETALA1 (ASAP1) and ASAPETALA3/TM6 (ASAP3/TM6) were studied in two species representing different lineages within the Hawaiian silversword alliance: Argyroxiphium sandwicense ssp. macrocephalum and Dubautia ciliolata ssp. glutinosa. Homoeologueous copies of ASAP1 and ASAP3/TM6 show differing levels and patterns of nucleotide polymorphism. Duplicate ASAP1 copies have similar levels of nucleotide diversity and haplotype structure in both species; by contrast, duplicate ASAP3/TM6 genes display different levels and patterns of variation in D. ciliolata ssp. glutinosa. Additionally, D. ciliolata ssp. glutinosa appears to be segregating for a moderate frequency null allele in one ASAP3/TM6 homoeologue. These results suggest that differing evolutionary forces can affect duplicate loci arising from allopolyploidization.

Original languageEnglish (US)
Pages (from-to)1301-1313
Number of pages13
JournalMolecular Ecology
Volume12
Issue number5
DOIs
StatePublished - May 1 2003

Fingerprint

Argyroxiphium
Asteraceae
Regulator Genes
regulator genes
Nucleotides
Genes
Duplicate Genes
nucleotides
duplicate genes
Polyploidy
gene
polyploidy
Argyroxiphium sandwicense
loci
Dubautia
genome
Genome
adaptive radiation
homeotic genes
allopolyploidy

Keywords

  • Adaptive radiation
  • Allopolyploidy
  • Floral homeotic genes
  • Gene duplication
  • MADS-box
  • Polyploidy

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Patterns of nucleotide variation in homoeologous regulatory genes in the allotetraploid Hawaiian silversword alliance (Asteraceae). / Lawton-Rauh, Amy; Robichaux, Robert H.; Purugganan, Michael D.

In: Molecular Ecology, Vol. 12, No. 5, 01.05.2003, p. 1301-1313.

Research output: Contribution to journalArticle

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