Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses

Roi Avraham, Nathan Haseley, Douglas Brown, Cristina Penaranda, Humberto B. Jijon, John J. Trombetta, Rahul Satija, Alex K. Shalek, Ramnik J. Xavier, Aviv Regev, Deborah T. Hung

Research output: Contribution to journalArticle

Abstract

Encounters between immune cells and invading bacteria ultimately determine the course of infection. These interactions are usually measured in populations of cells, masking cell-to-cell variation that may be important for infection outcome. To characterize the gene expression variation that underlies distinct infection outcomes and monitor infection phenotypes, we developed an experimental system that combines single-cell RNA-seq with fluorescent markers. Probing the responses of individual macrophages to invading Salmonella, we find that variation between individual infected host cells is determined by the heterogeneous activity of bacterial factors in individual infecting bacteria. We illustrate how variable PhoPQ activity in the population of invading bacteria drives variable host type I IFN responses by modifying LPS in a subset of bacteria. This work demonstrates a causative link between host and bacterial variability, with cell-to-cell variation between different bacteria being sufficient to drive radically different host immune responses. This co-variation has implications for host-pathogen dynamics in vivo.

Original languageEnglish (US)
Pages (from-to)1309-1321
Number of pages13
JournalCell
Volume162
Issue number6
DOIs
StatePublished - Jan 30 2015

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Pathogens
Bacteria
Infection
Salmonella
Macrophages
Gene expression
Cells
RNA
Population
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Avraham, R., Haseley, N., Brown, D., Penaranda, C., Jijon, H. B., Trombetta, J. J., ... Hung, D. T. (2015). Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. Cell, 162(6), 1309-1321. https://doi.org/10.1016/j.cell.2015.08.027

Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. / Avraham, Roi; Haseley, Nathan; Brown, Douglas; Penaranda, Cristina; Jijon, Humberto B.; Trombetta, John J.; Satija, Rahul; Shalek, Alex K.; Xavier, Ramnik J.; Regev, Aviv; Hung, Deborah T.

In: Cell, Vol. 162, No. 6, 30.01.2015, p. 1309-1321.

Research output: Contribution to journalArticle

Avraham, R, Haseley, N, Brown, D, Penaranda, C, Jijon, HB, Trombetta, JJ, Satija, R, Shalek, AK, Xavier, RJ, Regev, A & Hung, DT 2015, 'Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses', Cell, vol. 162, no. 6, pp. 1309-1321. https://doi.org/10.1016/j.cell.2015.08.027
Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ et al. Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. Cell. 2015 Jan 30;162(6):1309-1321. https://doi.org/10.1016/j.cell.2015.08.027
Avraham, Roi ; Haseley, Nathan ; Brown, Douglas ; Penaranda, Cristina ; Jijon, Humberto B. ; Trombetta, John J. ; Satija, Rahul ; Shalek, Alex K. ; Xavier, Ramnik J. ; Regev, Aviv ; Hung, Deborah T. / Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. In: Cell. 2015 ; Vol. 162, No. 6. pp. 1309-1321.
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