Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts

Flavia Q. Pirih, Jeanne Nervina, Lee Pham, Tara Aghaloo, Sotirios Tetradis

Research output: Contribution to journalArticle

Abstract

Parathyroid hormone (PTH) significantly affects osteoblast function by altering gene expression. We have identified neuron-derived orphan receptor-1 (NOR-1) as a PTH-induced primary gene in osteoblastic cells. NOR-1, Nurr1, and Nur77 comprise the NGFI-B nuclear orphan receptor family and Nurr1 and Nur77 are PTH-induced primary osteoblastic genes. Ten nM PTH maximally induced NOR-1 mRNA at 2h in primary mouse osteoblasts and at 1h in mouse calvariae. Cycloheximide pretreatment did not inhibit PTH-induced NOR-1 mRNA. PTH activates cAMP-protein kinase A (PKA), protein kinase C (PKC), and calcium signaling. Forskolin (PKA activator) and PMA (PKC activator) mimicked PTH-induced NOR-1 mRNA. Ionomycin (calcium ionophore) and PTH(3-34), which do not activate PKA, failed to induce NOR-1 mRNA. PKA inhibition with H89 blocked PTH- and FSK-induced NOR-1 mRNA. PMA pretreatment to deplete PKC inhibited PMA-induced, but not PTH-induced, NOR-1 mRNA. We conclude that NOR-1 is a PTH-regulated primary osteoblastic gene that is induced mainly through cAMP-PKA signaling.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
StatePublished - Jun 20 2003

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Nuclear Receptor Subfamily 4, Group A, Member 3
Orphan Nuclear Receptors
Osteoblasts
Parathyroid Hormone
Cyclic AMP-Dependent Protein Kinases
Messenger RNA
Protein Kinase C
Genes
Nuclear Receptor Subfamily 4, Group A, Member 1
Ionomycin
Frequency shift keying
Calcium Signaling
Calcium Ionophores
Colforsin
Cycloheximide
Skull
Gene expression

Keywords

  • NGFI-B
  • NOR-1
  • Orphan nuclear receptor
  • Osteoblast
  • PTH

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts. / Pirih, Flavia Q.; Nervina, Jeanne; Pham, Lee; Aghaloo, Tara; Tetradis, Sotirios.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 1, 20.06.2003, p. 144-150.

Research output: Contribution to journalArticle

Pirih, Flavia Q. ; Nervina, Jeanne ; Pham, Lee ; Aghaloo, Tara ; Tetradis, Sotirios. / Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 1. pp. 144-150.
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