Paradoxical surrogate markers of dental injury-induced pain in the mouse

Jennifer Gibbs, Rochelle Urban, Allan I. Basbaum

Research output: Contribution to journalArticle

Abstract

Dental pain, including toothache, is one of the most prevalent types of orofacial pain, causing severe, persistent pain that has a significant negative effect on quality of life, including eating disturbances, mood changes, and sleep disruption. As the primary cause of toothache pain is injury to the uniquely innervated dental pulp, rodent models of this injury provide the opportunity to study neurobiological mechanisms of tissue injury-induced persistent pain. Here we evaluated behavioral changes in mice with a dental pulp injury (DPI) produced by mechanically exposing the pulp to the oral environment. We monitored the daily life behaviors of mice with DPI, including measures of eating, drinking, and movement. During the first 48 hours, the only parameter affected by DPI was locomotion, which was reduced. There was also a significant short-term decrease in the amount of weight gained by DPI animals that was not related to food consumption. As cold allodynia is frequently observed in individuals experiencing toothache pain, we tested whether mice with DPI demonstrate an aversion to drinking cold liquids using a cold-sucrose consumption test. Surprisingly, mice with DPI increased their consumption of sucrose solution, to over 150% of baseline, regardless of temperature. Both the weight loss and increased sucrose intake in the first 2 days of injury were reversed by administration of indomethacin. These findings indicate that enhanced sucrose consumption may be a reliable measure of orofacial pain in rodents, and suggest that alterations in energy expenditure and motivational behaviors are under-recognized outcomes of tooth injury.

Original languageEnglish (US)
Pages (from-to)1358-1367
Number of pages10
JournalPain
Volume154
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Tooth Injuries
Dental Pulp
Biomarkers
Pain
Toothache
Sucrose
Facial Pain
Wounds and Injuries
Drinking
Rodentia
Eating
Hyperalgesia
Locomotion
Indomethacin
Energy Metabolism
Weight Loss
Tooth
Sleep
Quality of Life
Weights and Measures

Keywords

  • Dental pain
  • Nonevoked behavior
  • Orofacial pain
  • Pulpitis
  • Weight

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Paradoxical surrogate markers of dental injury-induced pain in the mouse. / Gibbs, Jennifer; Urban, Rochelle; Basbaum, Allan I.

In: Pain, Vol. 154, No. 8, 08.2013, p. 1358-1367.

Research output: Contribution to journalArticle

Gibbs, Jennifer ; Urban, Rochelle ; Basbaum, Allan I. / Paradoxical surrogate markers of dental injury-induced pain in the mouse. In: Pain. 2013 ; Vol. 154, No. 8. pp. 1358-1367.
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