Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores

Galina D. Mironova, Odile Gateau-Roesch, Christiane Levrat, Elena Gritsenko, Evgeny Pavlov, Alissa V. Lazareva, Elena Limarenko, Catherine Rey, Pierre Louisot, Nils Erik L Saris

Research output: Contribution to journalArticle

Abstract

A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in blacklipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain saturated fatty acids-mainly palmitic and stearic. These fatty acids, similar to the mitochondrial hydrophobic component, bind Ca2+ with high affinity in comparison with unsaturated fatty acids, saturated fatty acids with shorter aliphatic chains, phospholipids, and other lipids. Ca2+binding is inhibited by Mg2+ but not by K+. For palmitic acid, the Kd for Ca2+ was 5 μM at pH 8.5 and 15 μM at pH 7.5, with the Bmax of 0.48 ± 0.08 mmol/g. This corresponds to one Ca2+ ion for eight palmitic acid molecules. The data of IR spectroscopy confirm that Ca2+ does not form ionic bonds with palmitic and stearic acids under hydrophobic conditions. It has been found that in the presence of Ca2+, palmitic and stearic acids, but not unsaturated FFA induce a nonspecific permeability in black-lipid membranes. Addition of Ca2+ in order to induce the permeability transition, increases the extractable amount of palmitic and stearic acids, the effect being prevented by a phospholipase A2 inhibitor. The possible involvement of palmitic and stearic acids in the mitochondrial nonspecific permeability is discussed.

Original languageEnglish (US)
Pages (from-to)319-331
Number of pages13
JournalJournal of Bioenergetics and Biomembranes
Volume33
Issue number4
DOIs
StatePublished - 2001

Fingerprint

Stearic Acids
Membrane Lipids
Permeability
Fatty Acids
Palmitic Acid
Unsaturated Fatty Acids
Ion Channels
Phospholipids
Spectrum Analysis
Ions
Lipids
Membranes

Keywords

  • Black-lipid membrane
  • Calcium binding
  • CsA-insensitive pore
  • Ion channel
  • Mitochondria
  • Palmitic acid
  • Phospholipase A

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores. / Mironova, Galina D.; Gateau-Roesch, Odile; Levrat, Christiane; Gritsenko, Elena; Pavlov, Evgeny; Lazareva, Alissa V.; Limarenko, Elena; Rey, Catherine; Louisot, Pierre; Saris, Nils Erik L.

In: Journal of Bioenergetics and Biomembranes, Vol. 33, No. 4, 2001, p. 319-331.

Research output: Contribution to journalArticle

Mironova, Galina D. ; Gateau-Roesch, Odile ; Levrat, Christiane ; Gritsenko, Elena ; Pavlov, Evgeny ; Lazareva, Alissa V. ; Limarenko, Elena ; Rey, Catherine ; Louisot, Pierre ; Saris, Nils Erik L. / Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores. In: Journal of Bioenergetics and Biomembranes. 2001 ; Vol. 33, No. 4. pp. 319-331.
@article{f47da2b1a16a4a2d82979eca9fe32102,
title = "Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores",
abstract = "A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in blacklipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain saturated fatty acids-mainly palmitic and stearic. These fatty acids, similar to the mitochondrial hydrophobic component, bind Ca2+ with high affinity in comparison with unsaturated fatty acids, saturated fatty acids with shorter aliphatic chains, phospholipids, and other lipids. Ca2+binding is inhibited by Mg2+ but not by K+. For palmitic acid, the Kd for Ca2+ was 5 μM at pH 8.5 and 15 μM at pH 7.5, with the Bmax of 0.48 ± 0.08 mmol/g. This corresponds to one Ca2+ ion for eight palmitic acid molecules. The data of IR spectroscopy confirm that Ca2+ does not form ionic bonds with palmitic and stearic acids under hydrophobic conditions. It has been found that in the presence of Ca2+, palmitic and stearic acids, but not unsaturated FFA induce a nonspecific permeability in black-lipid membranes. Addition of Ca2+ in order to induce the permeability transition, increases the extractable amount of palmitic and stearic acids, the effect being prevented by a phospholipase A2 inhibitor. The possible involvement of palmitic and stearic acids in the mitochondrial nonspecific permeability is discussed.",
keywords = "Black-lipid membrane, Calcium binding, CsA-insensitive pore, Ion channel, Mitochondria, Palmitic acid, Phospholipase A",
author = "Mironova, {Galina D.} and Odile Gateau-Roesch and Christiane Levrat and Elena Gritsenko and Evgeny Pavlov and Lazareva, {Alissa V.} and Elena Limarenko and Catherine Rey and Pierre Louisot and Saris, {Nils Erik L}",
year = "2001",
doi = "10.1023/A:1010659323937",
language = "English (US)",
volume = "33",
pages = "319--331",
journal = "Journal of Bioenergetics and Biomembranes",
issn = "0145-479X",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores

AU - Mironova, Galina D.

AU - Gateau-Roesch, Odile

AU - Levrat, Christiane

AU - Gritsenko, Elena

AU - Pavlov, Evgeny

AU - Lazareva, Alissa V.

AU - Limarenko, Elena

AU - Rey, Catherine

AU - Louisot, Pierre

AU - Saris, Nils Erik L

PY - 2001

Y1 - 2001

N2 - A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in blacklipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain saturated fatty acids-mainly palmitic and stearic. These fatty acids, similar to the mitochondrial hydrophobic component, bind Ca2+ with high affinity in comparison with unsaturated fatty acids, saturated fatty acids with shorter aliphatic chains, phospholipids, and other lipids. Ca2+binding is inhibited by Mg2+ but not by K+. For palmitic acid, the Kd for Ca2+ was 5 μM at pH 8.5 and 15 μM at pH 7.5, with the Bmax of 0.48 ± 0.08 mmol/g. This corresponds to one Ca2+ ion for eight palmitic acid molecules. The data of IR spectroscopy confirm that Ca2+ does not form ionic bonds with palmitic and stearic acids under hydrophobic conditions. It has been found that in the presence of Ca2+, palmitic and stearic acids, but not unsaturated FFA induce a nonspecific permeability in black-lipid membranes. Addition of Ca2+ in order to induce the permeability transition, increases the extractable amount of palmitic and stearic acids, the effect being prevented by a phospholipase A2 inhibitor. The possible involvement of palmitic and stearic acids in the mitochondrial nonspecific permeability is discussed.

AB - A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in blacklipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain saturated fatty acids-mainly palmitic and stearic. These fatty acids, similar to the mitochondrial hydrophobic component, bind Ca2+ with high affinity in comparison with unsaturated fatty acids, saturated fatty acids with shorter aliphatic chains, phospholipids, and other lipids. Ca2+binding is inhibited by Mg2+ but not by K+. For palmitic acid, the Kd for Ca2+ was 5 μM at pH 8.5 and 15 μM at pH 7.5, with the Bmax of 0.48 ± 0.08 mmol/g. This corresponds to one Ca2+ ion for eight palmitic acid molecules. The data of IR spectroscopy confirm that Ca2+ does not form ionic bonds with palmitic and stearic acids under hydrophobic conditions. It has been found that in the presence of Ca2+, palmitic and stearic acids, but not unsaturated FFA induce a nonspecific permeability in black-lipid membranes. Addition of Ca2+ in order to induce the permeability transition, increases the extractable amount of palmitic and stearic acids, the effect being prevented by a phospholipase A2 inhibitor. The possible involvement of palmitic and stearic acids in the mitochondrial nonspecific permeability is discussed.

KW - Black-lipid membrane

KW - Calcium binding

KW - CsA-insensitive pore

KW - Ion channel

KW - Mitochondria

KW - Palmitic acid

KW - Phospholipase A

UR - http://www.scopus.com/inward/record.url?scp=0034750552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034750552&partnerID=8YFLogxK

U2 - 10.1023/A:1010659323937

DO - 10.1023/A:1010659323937

M3 - Article

VL - 33

SP - 319

EP - 331

JO - Journal of Bioenergetics and Biomembranes

JF - Journal of Bioenergetics and Biomembranes

SN - 0145-479X

IS - 4

ER -