Organ regeneration does not require a functional stem cell niche in plants

Giovanni Sena, Xiaoning Wang, Hsiao Yun Liu, Hugo Hofhuis, Kenneth D. Birnbaum

Research output: Contribution to journalArticle

Abstract

Plants rely on the maintenance of stem cell niches at their apices for the continuous growth of roots and shoots. However, although the developmental plasticity of plant cells has been demonstrated, it is not known whether the stem cell niche is required for organogenesis. Here we explore the capacity of a broad range of differentiating cells to regenerate an organ without the activity of a stem cell niche. Using a root-tip regeneration system in Arabidopsis thaliana to track the molecular and functional recovery of cell fates, we show that re-specification of lost cell identities begins within hours of excision and that the function of specialized cells is restored within one day. Critically, regeneration proceeds in plants with mutations that fail to maintain the stem cell niche. These results show that stem-cell-like properties that mediate complete organ regeneration are dispersed in plant meristems and are not restricted to niches, which nonetheless seem to be necessary for indeterminate growth. This regenerative reprogramming of an entire organ without transition to a stereotypical stem cell environment has intriguing parallels to recent reports of induced transdifferentiation of specific cell types in the adult organs of animals.

Original languageEnglish (US)
Pages (from-to)1150-1153
Number of pages4
JournalNature
Volume457
Issue number7233
DOIs
StatePublished - Feb 26 2009

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Stem Cell Niche
Regeneration
Meristem
Stem Cells
Cell Transdifferentiation
Animal Structures
Organogenesis
Plant Cells
Growth
Arabidopsis
Maintenance
Mutation

ASJC Scopus subject areas

  • General

Cite this

Organ regeneration does not require a functional stem cell niche in plants. / Sena, Giovanni; Wang, Xiaoning; Liu, Hsiao Yun; Hofhuis, Hugo; Birnbaum, Kenneth D.

In: Nature, Vol. 457, No. 7233, 26.02.2009, p. 1150-1153.

Research output: Contribution to journalArticle

Sena, Giovanni ; Wang, Xiaoning ; Liu, Hsiao Yun ; Hofhuis, Hugo ; Birnbaum, Kenneth D. / Organ regeneration does not require a functional stem cell niche in plants. In: Nature. 2009 ; Vol. 457, No. 7233. pp. 1150-1153.
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