Oral vs. salivary diagnostics

Joana Marques, Patricia M. Corby, Cheryl A. Barber, William R. Abrams, Daniel Malamud

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The field ofsalivary diagnostics€ includes studies utilizing samples obtained from a variety of sources within the oral cavity. These samples include; whole unstimulated saliva, stimulated whole saliva, duct saliva collected directly from the parotid, submandibular/sublingual glands or minor salivary glands, swabs of the buccal mucosa, tongue or tonsils, and gingival crevicular fluid. Many publications statewe collected saliva from subjects€ without fully describing the process or source of the oral fluid. Factors that need to be documented in any study include the time of day of the collection, the method used to stimulate and collect the fluid, and how much fluid is being collected and for how long. The handling of the oral fluid during and post-collection is also critical and may include addition of protease or nuclease inhibitors, centrifugation, and cold or frozen storage prior to assay. In an effort to create a standard protocol for determining a biomarker€™s origin we carried out a pilot study collecting oral fluid from 5 different sites in the mouth and monitoring the concentrations of pro- and anti-inflammatory cytokines detected using MesoScaleDiscovery (MSD) electrochemiluminesence assays. Our data suggested that 3 of the cytokines are primarily derived from the submandibular gland, while 7 of the cytokines come from a source other than the major salivary glands such as the minor salivary glands or cells in the oral mucosae. Here we review the literature on monitoring biomarkers in oral samples and stress the need for determining the blood/saliva ratio when a quantitative determination is needed and suggest that the term oral diagnostic be used if the source of an analyte in the oral cavity is unknown.

Original languageEnglish (US)
Title of host publicationAdvances in Global Health Through Sensing Technologies 2015
PublisherSPIE
Volume9490
ISBN (Print)9781628416060
DOIs
StatePublished - 2015
EventAdvances in Global Health Through Sensing Technologies 2015 - Baltimore, United States
Duration: Apr 20 2015Apr 21 2015

Other

OtherAdvances in Global Health Through Sensing Technologies 2015
CountryUnited States
CityBaltimore
Period4/20/154/21/15

Fingerprint

saliva
Diagnostics
salivary glands
Fluid
Cytokines
Fluids
fluids
glands
biomarkers
Biomarkers
Minor
Assays
Cavity
Monitoring
nuclease
protease
cavities
tongue
Centrifugation
mouth

Keywords

  • Cytokines
  • Diagnostic
  • Oral fluid
  • Saliva

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Marques, J., Corby, P. M., Barber, C. A., Abrams, W. R., & Malamud, D. (2015). Oral vs. salivary diagnostics. In Advances in Global Health Through Sensing Technologies 2015 (Vol. 9490). [949004] SPIE. https://doi.org/10.1117/12.2183327

Oral vs. salivary diagnostics. / Marques, Joana; Corby, Patricia M.; Barber, Cheryl A.; Abrams, William R.; Malamud, Daniel.

Advances in Global Health Through Sensing Technologies 2015. Vol. 9490 SPIE, 2015. 949004.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Marques, J, Corby, PM, Barber, CA, Abrams, WR & Malamud, D 2015, Oral vs. salivary diagnostics. in Advances in Global Health Through Sensing Technologies 2015. vol. 9490, 949004, SPIE, Advances in Global Health Through Sensing Technologies 2015, Baltimore, United States, 4/20/15. https://doi.org/10.1117/12.2183327
Marques J, Corby PM, Barber CA, Abrams WR, Malamud D. Oral vs. salivary diagnostics. In Advances in Global Health Through Sensing Technologies 2015. Vol. 9490. SPIE. 2015. 949004 https://doi.org/10.1117/12.2183327
Marques, Joana ; Corby, Patricia M. ; Barber, Cheryl A. ; Abrams, William R. ; Malamud, Daniel. / Oral vs. salivary diagnostics. Advances in Global Health Through Sensing Technologies 2015. Vol. 9490 SPIE, 2015.
@inproceedings{7ace84116016441d8bda52e479adb5c8,
title = "Oral vs. salivary diagnostics",
abstract = "The field ofsalivary diagnostics€ includes studies utilizing samples obtained from a variety of sources within the oral cavity. These samples include; whole unstimulated saliva, stimulated whole saliva, duct saliva collected directly from the parotid, submandibular/sublingual glands or minor salivary glands, swabs of the buccal mucosa, tongue or tonsils, and gingival crevicular fluid. Many publications statewe collected saliva from subjects€ without fully describing the process or source of the oral fluid. Factors that need to be documented in any study include the time of day of the collection, the method used to stimulate and collect the fluid, and how much fluid is being collected and for how long. The handling of the oral fluid during and post-collection is also critical and may include addition of protease or nuclease inhibitors, centrifugation, and cold or frozen storage prior to assay. In an effort to create a standard protocol for determining a biomarker€™s origin we carried out a pilot study collecting oral fluid from 5 different sites in the mouth and monitoring the concentrations of pro- and anti-inflammatory cytokines detected using MesoScaleDiscovery (MSD) electrochemiluminesence assays. Our data suggested that 3 of the cytokines are primarily derived from the submandibular gland, while 7 of the cytokines come from a source other than the major salivary glands such as the minor salivary glands or cells in the oral mucosae. Here we review the literature on monitoring biomarkers in oral samples and stress the need for determining the blood/saliva ratio when a quantitative determination is needed and suggest that the term oral diagnostic be used if the source of an analyte in the oral cavity is unknown.",
keywords = "Cytokines, Diagnostic, Oral fluid, Saliva",
author = "Joana Marques and Corby, {Patricia M.} and Barber, {Cheryl A.} and Abrams, {William R.} and Daniel Malamud",
year = "2015",
doi = "10.1117/12.2183327",
language = "English (US)",
isbn = "9781628416060",
volume = "9490",
booktitle = "Advances in Global Health Through Sensing Technologies 2015",
publisher = "SPIE",

}

TY - GEN

T1 - Oral vs. salivary diagnostics

AU - Marques, Joana

AU - Corby, Patricia M.

AU - Barber, Cheryl A.

AU - Abrams, William R.

AU - Malamud, Daniel

PY - 2015

Y1 - 2015

N2 - The field ofsalivary diagnostics€ includes studies utilizing samples obtained from a variety of sources within the oral cavity. These samples include; whole unstimulated saliva, stimulated whole saliva, duct saliva collected directly from the parotid, submandibular/sublingual glands or minor salivary glands, swabs of the buccal mucosa, tongue or tonsils, and gingival crevicular fluid. Many publications statewe collected saliva from subjects€ without fully describing the process or source of the oral fluid. Factors that need to be documented in any study include the time of day of the collection, the method used to stimulate and collect the fluid, and how much fluid is being collected and for how long. The handling of the oral fluid during and post-collection is also critical and may include addition of protease or nuclease inhibitors, centrifugation, and cold or frozen storage prior to assay. In an effort to create a standard protocol for determining a biomarker€™s origin we carried out a pilot study collecting oral fluid from 5 different sites in the mouth and monitoring the concentrations of pro- and anti-inflammatory cytokines detected using MesoScaleDiscovery (MSD) electrochemiluminesence assays. Our data suggested that 3 of the cytokines are primarily derived from the submandibular gland, while 7 of the cytokines come from a source other than the major salivary glands such as the minor salivary glands or cells in the oral mucosae. Here we review the literature on monitoring biomarkers in oral samples and stress the need for determining the blood/saliva ratio when a quantitative determination is needed and suggest that the term oral diagnostic be used if the source of an analyte in the oral cavity is unknown.

AB - The field ofsalivary diagnostics€ includes studies utilizing samples obtained from a variety of sources within the oral cavity. These samples include; whole unstimulated saliva, stimulated whole saliva, duct saliva collected directly from the parotid, submandibular/sublingual glands or minor salivary glands, swabs of the buccal mucosa, tongue or tonsils, and gingival crevicular fluid. Many publications statewe collected saliva from subjects€ without fully describing the process or source of the oral fluid. Factors that need to be documented in any study include the time of day of the collection, the method used to stimulate and collect the fluid, and how much fluid is being collected and for how long. The handling of the oral fluid during and post-collection is also critical and may include addition of protease or nuclease inhibitors, centrifugation, and cold or frozen storage prior to assay. In an effort to create a standard protocol for determining a biomarker€™s origin we carried out a pilot study collecting oral fluid from 5 different sites in the mouth and monitoring the concentrations of pro- and anti-inflammatory cytokines detected using MesoScaleDiscovery (MSD) electrochemiluminesence assays. Our data suggested that 3 of the cytokines are primarily derived from the submandibular gland, while 7 of the cytokines come from a source other than the major salivary glands such as the minor salivary glands or cells in the oral mucosae. Here we review the literature on monitoring biomarkers in oral samples and stress the need for determining the blood/saliva ratio when a quantitative determination is needed and suggest that the term oral diagnostic be used if the source of an analyte in the oral cavity is unknown.

KW - Cytokines

KW - Diagnostic

KW - Oral fluid

KW - Saliva

UR - http://www.scopus.com/inward/record.url?scp=84943368828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943368828&partnerID=8YFLogxK

U2 - 10.1117/12.2183327

DO - 10.1117/12.2183327

M3 - Conference contribution

AN - SCOPUS:84943368828

SN - 9781628416060

VL - 9490

BT - Advances in Global Health Through Sensing Technologies 2015

PB - SPIE

ER -