Optochemical genetics

Timm Fehrentz, Matthias Schönberger, Dirk Trauner

Research output: Contribution to journalReview article

Abstract

Transmembrane receptors allow a cell to communicate with its environment in response to a variety of input signals. These can be changes in the concentration of ligands (e.g. hormones or neurotransmitters), temperature, pressure (e.g. acoustic waves or touch), transmembrane potential, or light intensity. Many important receptors have now been characterized in atomic detail and our understanding of their functional properties has markedly increased in recent years. As a consequence, these sophisticated molecular machines can be reprogrammed to respond to unnatural input signals. In this Review, we show how voltage-gated and ligand-gated ion channels can be endowed with synthetic photoswitches, and how the resulting artificial photoreceptors can be used to optically control neurons with exceptional temporal and spatial precision. They work well in animals and might find applications in the restoration of vision and the optical control of other sensations. The combination of synthetic photoswitches and receptor proteins contributes to the field of optogenetics and adds a new functional dimension to chemical genetics. As such, we propose to call it "optochemical genetics". Light of my life: The merger of natural transmembrane proteins with synthetic photoswitches creates hybrid receptors that can be integrated into complex systems and regulated with the precision that only light provides. This strategy allows for the optical control of single cells, neural systems, and can even be used to control animal behavior.

Original languageEnglish (US)
Pages (from-to)12156-12182
Number of pages27
JournalAngewandte Chemie - International Edition
Volume50
Issue number51
DOIs
StatePublished - Dec 16 2011

Fingerprint

Animals
Ligands
Artificial Receptors
Ligand-Gated Ion Channels
Proteins
Hormones
Restoration
Neurons
Neurotransmitter Agents
Large scale systems
Acoustic waves
Genetics
Ions
Electric potential
Temperature

Keywords

  • chemical genetics
  • ion channels
  • optogenetics
  • photoswitches
  • receptors

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Fehrentz, T., Schönberger, M., & Trauner, D. (2011). Optochemical genetics. Angewandte Chemie - International Edition, 50(51), 12156-12182. https://doi.org/10.1002/anie.201103236

Optochemical genetics. / Fehrentz, Timm; Schönberger, Matthias; Trauner, Dirk.

In: Angewandte Chemie - International Edition, Vol. 50, No. 51, 16.12.2011, p. 12156-12182.

Research output: Contribution to journalReview article

Fehrentz, T, Schönberger, M & Trauner, D 2011, 'Optochemical genetics', Angewandte Chemie - International Edition, vol. 50, no. 51, pp. 12156-12182. https://doi.org/10.1002/anie.201103236
Fehrentz T, Schönberger M, Trauner D. Optochemical genetics. Angewandte Chemie - International Edition. 2011 Dec 16;50(51):12156-12182. https://doi.org/10.1002/anie.201103236
Fehrentz, Timm ; Schönberger, Matthias ; Trauner, Dirk. / Optochemical genetics. In: Angewandte Chemie - International Edition. 2011 ; Vol. 50, No. 51. pp. 12156-12182.
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