Optical tools for understanding the complexity of β-cell signalling and insulin release

James A. Frank, Johannes Broichhagen, Dmytro A. Yushchenko, Dirk Trauner, Carsten Schultz, David J. Hodson

Research output: Contribution to journalArticle

Abstract

Following stimulation, pancreatic β-cells must orchestrate a plethora of signalling events to ensure the appropriate release of insulin and maintenance of normal glucose homeostasis. Failure at any point in this cascade leads to impaired insulin secretion, elevated blood levels of glucose and eventually type 2 diabetes mellitus. Likewise, β-cell replacement or regeneration strategies for the treatment of both type 1 and type 2 diabetes mellitus might fail if the correct cell signalling phenotype cannot be faithfully recreated. However, current understanding of β-cell function is complicated because of the highly dynamic nature of their intracellular and intercellular signalling as well as insulin release itself. β-Cells must precisely integrate multiple signals stemming from multiple cues, often with differing intensities, frequencies and cellular and subcellular localizations, before converging these signals onto insulin exocytosis. In this respect, optical approaches with high resolution in space and time are extremely useful for properly deciphering the complexity of β-cell signalling. An increased understanding of β-cell signalling might identify new mechanisms underlying insulin release, with relevance for future drug therapy and de novo stem cell engineering of functional islets.

Original languageEnglish (US)
JournalNature Reviews Endocrinology
DOIs
StateAccepted/In press - Jan 1 2018

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Insulin
Type 2 Diabetes Mellitus
Cell Engineering
Exocytosis
Type 1 Diabetes Mellitus
Cues
Blood Glucose
Regeneration
Homeostasis
Stem Cells
Maintenance
Phenotype
Glucose
Drug Therapy
Therapeutics

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Frank, J. A., Broichhagen, J., Yushchenko, D. A., Trauner, D., Schultz, C., & Hodson, D. J. (Accepted/In press). Optical tools for understanding the complexity of β-cell signalling and insulin release. Nature Reviews Endocrinology. https://doi.org/10.1038/s41574-018-0105-2

Optical tools for understanding the complexity of β-cell signalling and insulin release. / Frank, James A.; Broichhagen, Johannes; Yushchenko, Dmytro A.; Trauner, Dirk; Schultz, Carsten; Hodson, David J.

In: Nature Reviews Endocrinology, 01.01.2018.

Research output: Contribution to journalArticle

Frank, James A. ; Broichhagen, Johannes ; Yushchenko, Dmytro A. ; Trauner, Dirk ; Schultz, Carsten ; Hodson, David J. / Optical tools for understanding the complexity of β-cell signalling and insulin release. In: Nature Reviews Endocrinology. 2018.
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