Optical Control of Lipid Rafts with Photoswitchable Ceramides

James Allen Frank, Henri G. Franquelim, Petra Schwille, Dirk Trauner

Research output: Contribution to journalArticle

Abstract

Ceramide is a pro-apoptotic sphingolipid with unique physical characteristics. Often viewed as a second messenger, its generation can modulate the structure of lipid rafts. We prepared three photoswitchable ceramides, ACes, which contain an azobenzene photoswitch allowing for optical control over the N-acyl chain. Using combined atomic force and confocal fluorescence microscopy, we demonstrate that the ACes enable reversible switching of lipid domains in raft-mimicking supported lipid bilayers (SLBs). In the trans-configuration, the ACes localize into the liquid-ordered (Lo) phase. Photoisomerization to the cis-form triggers a fluidification of the Lo domains, as liquid-disordered (Ld) "lakes" are formed within the rafts. Photoisomerization back to the trans-state with blue light stimulates a rigidification inside the Ld phase, as the formation of small Lo domains. These changes can be repeated over multiple cycles, enabling a dynamic spatiotemporal control of the lipid raft structure with light.

Original languageEnglish (US)
Pages (from-to)12981-12986
Number of pages6
JournalJournal of the American Chemical Society
Volume138
Issue number39
DOIs
StatePublished - Oct 5 2016

Fingerprint

Ceramides
Lipids
Liquids
Photoisomerization
Light
Sphingolipids
Lipid Bilayers
Second Messenger Systems
Lakes
Fluorescence Microscopy
Confocal Microscopy
Lipid bilayers
Azobenzene
Confocal microscopy
Fluorescence microscopy

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Frank, J. A., Franquelim, H. G., Schwille, P., & Trauner, D. (2016). Optical Control of Lipid Rafts with Photoswitchable Ceramides. Journal of the American Chemical Society, 138(39), 12981-12986. https://doi.org/10.1021/jacs.6b07278

Optical Control of Lipid Rafts with Photoswitchable Ceramides. / Frank, James Allen; Franquelim, Henri G.; Schwille, Petra; Trauner, Dirk.

In: Journal of the American Chemical Society, Vol. 138, No. 39, 05.10.2016, p. 12981-12986.

Research output: Contribution to journalArticle

Frank, JA, Franquelim, HG, Schwille, P & Trauner, D 2016, 'Optical Control of Lipid Rafts with Photoswitchable Ceramides', Journal of the American Chemical Society, vol. 138, no. 39, pp. 12981-12986. https://doi.org/10.1021/jacs.6b07278
Frank, James Allen ; Franquelim, Henri G. ; Schwille, Petra ; Trauner, Dirk. / Optical Control of Lipid Rafts with Photoswitchable Ceramides. In: Journal of the American Chemical Society. 2016 ; Vol. 138, No. 39. pp. 12981-12986.
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