Optical control of GIRK channels using visible light

Julie B. Trads, Jessica Burgstaller, Laura Laprell, David B. Konrad, Luis De La Osa de La Rosa, C. David Weaver, Herwig Baier, Dirk Trauner, David M. Barber

Research output: Contribution to journalArticle

Abstract

G-protein coupled inwardly rectifying potassium (GIRK) channels are an integral part of inhibitory signal transduction pathways, reducing the activity of excitable cells via hyperpolarization. They play crucial roles in processes such as cardiac output, cognition and the coordination of movement. Therefore, the precision control of GIRK channels is of critical importance. Here, we describe the development of the azobenzene containing molecule VLOGO (Visible Light Operated GIRK channel Opener), which activates GIRK channels in the dark and is promptly deactivated when illuminated with green light. VLOGO is a valuable addition to the existing tools for the optical control of GIRK channels as it circumvents the need to use potentially harmful UV irradiation. We therefore believe that VLOGO will be a useful research tool for studying GIRK channels in biological systems.

Original languageEnglish (US)
Pages (from-to)76-81
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2017

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ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Trads, J. B., Burgstaller, J., Laprell, L., Konrad, D. B., De La Osa de La Rosa, L., Weaver, C. D., Baier, H., Trauner, D., & Barber, D. M. (2017). Optical control of GIRK channels using visible light. Organic and Biomolecular Chemistry, 15(1), 76-81. https://doi.org/10.1039/c6ob02153k